کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
170986 458426 2012 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A thienoquinoxaline and a styryl-quinoxaline as new fluorescent probes for amyloid-β fibrils
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
A thienoquinoxaline and a styryl-quinoxaline as new fluorescent probes for amyloid-β fibrils
چکیده انگلیسی

Simple and easy to prepare quinoxaline derivatives proved able to stain amyloid fibers such as aggregated lysozyme and Aβ(1-40)-peptide by a fluorescence “turn on” mechanism. Thienoquinoxaline 1 allowed the detection of lysozyme and Aβ(1-40) fibers at λ = 555 and 532 nm, respectively, with excitation at λ = 450 nm. Styryl-quinoxaline 2 stained lysozyme and Aβ(1-40) fibers with fluorescence at λ = 579 and 567 nm, respectively, upon excitation at λ = 453 nm. The apparent Kd values for Aβ(1-40) fibers were 77 and 294 nM for 1 and 2, respectively. The sensitivity of the aggregates detection assay with these new dyes was higher than that of thioflavin T. Considering their unique fluorescence properties compared to other dyes reported in the field, they can be considered as additional staining tools for the detection and studies of peptide/protein aggregation.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comptes Rendus Chimie - Volume 15, Issue 1, January 2012, Pages 79–85
نویسندگان
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