کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
175414 458909 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Fluorescent bioinspired protein labeling with betalamic acid. Derivatization and characterization of novel protein-betaxanthins
ترجمه فارسی عنوان
برچسب زدن پروتئین فلورسنت زیستی با اسید بتالامیک. تشخیص و تعریف پروتئین های بتاکانتانین جدید
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
چکیده انگلیسی


• Proteins are labeled with natural pigment betalamic acid for the first time.
• Novel protein-betaxanthins are fluorescent with visible light.
• Labeled proteins can be detected in electrophoresis by conventional imaging systems.
• Labeling can be started from purified betalamic acid or raw red beet root extracts.

Betaxanthins are the water-soluble pigments that bestow yellow coloration to fruits, flowers and roots of plants of the Caryophyllales order and present autofluorescence after excitation with blue light. In this work, the semi-synthesis of betaxanthins derived from macromolecules is achieved for the first time by exploiting the reactivity of amine groups belonging to proteins. The synthesis of protein-betaxanthins is demonstrated by spectrophotometry and HPLC-ESI-TOF-MS mass analysis. The derivatization with betalamic acid was in a ratio 1:1 and yielded protein-betaxanthins yellow in color that exhibited fluorescent properties with a maximum excitation wavelength of 476 nm and a maximum emission wavelength of 551 nm. Moreover, staining can be started from purified betalamic acid or directly from raw red beet root extracts. The novel bioinspired labeling reaction allowed protein detection in conventional fluorescence scanning and imaging systems and opens a new perspective for betalamic acid derived molecules as fluorescent probes with multiple biological applications.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 133, October 2016, Pages 458–466
نویسندگان
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