Controlled assemble of erythrosine B/layered double hydroxide ultrathin film: Preparation, fluorescence properties, and photo-response to bovine serum albumin

• The ER/LDH UTFs were fabricated through layer-by-layer assemble.
• The ER/LDH UTFs display a uniform morphology and a periodical layered structure.
• The (ER/LDH)26 UTF exhibits a good fluorescence response to BSA.
• The UTF nanocomposites may be created for adsorption and separation of biomolecules, drug delivery, and molecular recognition.

The ultrathin films (UTFs) based on erythrosine B/layered double hydroxide have been prepared by a layer-by-layer assembly technique. UV–vis, fluorescence spectroscopy, XRD, SEM and AFM have been adopted to monitor the assembly process. The UTFs display a uniform morphology and a periodical layered structure. Fluorescence spectra demonstrated that the (ER/LDH)26 UTF exhibited the optimal luminescent intensity. Moreover, the fluorescence-response of (ER/LDH)26 UTF to bovine serum albumin (BSA) was investigated. The films have a good selectivity and reusable ability. The specific fluorescence response of the UTF is attributed to a strong interaction between ER and BSA. Circular dichroism (CD) revealed that the secondary conformation of BSA has been changed from α-helical structure to β-sheet after adsorbed on (ER/LDH)26 UTF, further confirmed the interaction between BSA and ER/LDH film. These results demonstrate that ER/LDH system can serve as a good candidate for the solid-sate luminescence and sensor materials for BSA.

The ultrathin films (UTFs) based on erythrosine dye/layered double hydroxide (ER/LDH) have been prepared by a layer-by-layer assembly technique. The UTFs display a uniform morphology and a periodical layered structure. Then the fluorescence-response of the UTFs to bovine serum albumin (BSA) has been investigated, which exhibits a good fluorescent response, high selectivity and recycle ability for long-term employment. The results indicate that, through engineering the ER/LDH system, the UTF nanocomposites may be created for adsorption and separation of biomolecules, drug delivery, and molecular recognition.Figure optionsDownload as PowerPoint slide