Application of ultrasound in preparing pathological sections to reduce processing time

It has been found experimentally that application of sub-mega and low megahertz ultrasound (US) of spatial and temporal averaged intensity Isata up to 10 W/cm2 during the process of preparing pathological sections of the mouse tissue has shortened the processing time from 12 h (without US) to less than half an hour (with US). The experiment has also showed that the processing time reached the shortest for ultrasound f = 200 kHz among the frequencies of 200 kHz, 400 kHz, 600 kHz, 800 kHz and 1 MHz used in this study. It has been proposed that ultrasound inducing non-inertial cavitation enhanced the permeability of cell membrane to liquid. Thus tissue fixation and dehydration were speeded up by application of US.