The effect of perspiration on photo-induced chemical reaction of azo dyes and the determination of aromatic amine products

In order to investigate the impact of perspiration on photo-induced chemical reaction of azo dyes and the carcinogenic aromatic amine products produced from the reaction, we have carried out experimental studies on the photochemical reaction of C.I. Reactive Red 2 mixed with American Association of Textile Chemists and Colorists（AATCC）standard artificial perspiration. UV–vis spectroscopic technique was employed to monitor the reaction processes, and the reaction products were analyzed by hollow fiber protected liquid-liquid-liquid phase micro-extraction with capillary electrophoresis（HF-LLLME-CE). The results showed that perspiration had remarkable influence on the photochemical reaction of azo dyes. Aromtic amines formed during the photochemical process as a result of reduction of azo dyes by organic components in perspiration. The HF-LLLME-CE methodology was validated in analyzing aromatic amines produced from the photochemical degradation of azo dye C.I. Reactive Red 2 and C.I. Acid Red 35 mixed with artificial perspiration.

► The synergistic effect of perspiration on photo-induced degradation of azo dyes was investigated. Organic components of perspiration and light intensity show remarkable affect. Inorganic salts in perspiration have inhibiting function. Employment of HF-LLLPME-CE realized the determination of aromatic amines in perspiration. Aniline and O-toluidine generated from azo dyes under photo-perspiration system were determined.