کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
177343 | 458978 | 2011 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Sol–gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Sol–gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye Sol–gel encapsulated glucose oxidase arrays based on a pH sensitive fluorescent dye](/preview/png/177343.png)
چکیده انگلیسی
Optical glucose oxidase (GOx) arrays based on pH sensitive fluorescent dye (2-(4-tolyl)-4-[4-(1,4,7,10-tetraoxa-13-azacyclopentadecyl)benzylidene]-5-oxazolone) (CPO) has been constructed. The arrays were prepared by spotting of CPO and GOx together with tetraethoxysilane (TEOS)/Chitosan (CHIT) mixture via a microarrayer. After optimization studies, analytical characterization of enzyme arrays were carried out. The fluorescence intensity of the system was linearly correlated to glucose concentration in the range of 1.0–30.0 mM (in potassium phosphate buffer; 2.5 mM at pH 7.0). Furthermore, the developed arrays were used to analyze glucose in some beverages and HPLC was used as a reference method for independent glucose analysis.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Dyes and Pigments - Volume 89, Issue 2, May 2011, Pages 144–148
Journal: Dyes and Pigments - Volume 89, Issue 2, May 2011, Pages 144–148
نویسندگان
Nimet Yildirim, Dilek Odaci, Gulsiye Ozturk, Serap Alp, Yavuz Ergun, Kay Dornbusch, Karl-Heinz Feller, Suna Timur,