The removal of C.I. Basic Red 46 in a mixed methanogenic anaerobic culture

The removal of C.I. Basic Red 46 (BR 46) was investigated with anaerobic mixed culture using glucose (3000 mg l−1 COD) as carbon source and electron donor. Zero-, first- and second-order reaction kinetics were used to find out the suitable substrate removal and decolorization kinetics. The substrate removal (COD) process is suitable for first-order reaction kinetic among the kinetic models studied. Increases in dye concentrations from 0 to 1000 mg l−1 decrease the degradation rate constant (k1) values from 0.0083 to 0.0032 h−1 in batch experiments carried out with BR 46. Decolorization process approximates to first-order kinetic at 50, 100 and 250 mg l−1 of BR 46, and to zero-order kinetic at 500, 750, 1000 mg l−1 of BR 46 concentration. Substrate and color removal rates (mg l−1 h−1) were found to be 8.07, 7.78, 7.64, 7.23, 6.39, 5.98, 5.28 and 0.66, 1.37, 3.48, 4.95, 6.30, 5.99, respectively, in all serum bottles throughout incubation period.