کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1788158 1023462 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Construction of biosensor for determination of galactose with galactose oxidase immobilized on polymeric mediator contains ferrocene
موضوعات مرتبط
مهندسی و علوم پایه فیزیک و نجوم فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
Construction of biosensor for determination of galactose with galactose oxidase immobilized on polymeric mediator contains ferrocene
چکیده انگلیسی

A galactose biosensor was developed by immobilizing galactose oxidase (GAox) on poly (glycidyl methacrylate-co-vinylferrocene) (poly (GMA-co-VFc)) film. The enzyme was immobilized via amine group onto polymeric mediator. The biosensor was characterized by the specific activity of the immobilized galactose oxidase, the apparent Michaelis constant KMapp, and the stability expressed by time and a number of the performed analysis. The optimized galactose biosensor exhibited a linear response range from 2 to 20 M and detection limit of 0.1 mM toward galactose. The response time of the biosensor was 5 s. The effects of the pH and the temperature of the immobilized galactose oxidase electrode were also studied.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Applied Physics - Volume 10, Issue 5, September 2010, Pages 1313–1316
نویسندگان
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