کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1788945 | 1023484 | 2009 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Neurotransmitter detection by enzyme-immobilized CNT-FET
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
فیزیک و نجوم
فیزیک ماده چگال
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
In order to detect l-glutamate, we immobilized l-glutamate oxidase (GLOD) on carbon nanotube (CNT) network junctions by a non-covalent functionalized method to preserve their electronic characteristics. After immobilizing GLOD on the CNT network, the excess reactive groups of linker molecule remaining on its surface were deactivated and blocked by ethanolamine. The electrical property of the GLOD-immobilized CNT network transistor was characterized as source-drain current that depended on liquid gate voltage. From the changes in the Isd vs. Vg plot about the CNT network transistor before and after the immobilization of GLOD, we confirmed that GLOD immobilization decreased the conductance of CNT network junctions, regardless of the sign on the net charge in the protein. The real time electronic response of the GLOD-coated CNT network transistor was conducted with a glutamate standard solution in vitro and the 11 vessel occlusion (11VO) rat model in vivo. The ultrahigh sensitivity, selectivity, and fast response time of GLOD-immobilized CNT-FET could provide great potential for the real time electronic detection of extracellular glutamate levels in the brain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Current Applied Physics - Volume 9, Issue 1, Supplement, January 2009, Pages S25-S28
Journal: Current Applied Physics - Volume 9, Issue 1, Supplement, January 2009, Pages S25-S28
نویسندگان
G.J. Lee, J.E. Lim, J.H. Park, S.K. Choi, S. Hong, H.K. Park,