Electro-generated reactive oxygen species at Au surface as an indicator to explore glutathione redox chemistry and quantification

• Conversion of GSH to GSSG by electrogenerated ROS was highlighted.
• Two reaction pathways for the GSH reaction depending upon [GSH] were characterized.
• Role of the presence of dissolved oxygen in trace level was identified.
• Presence of higher dissolved oxygen affects the sensitivity of GSH determination.
• Determination of ‘total GSH’ by electro-reduction of GSSG on Au was achieved.

The thiol to disulfide inter-conversion plays vital role in the glutathione (GSH) metabolism in human body. Mimicking the GSH conversion to disulfide by electro-generated reactive oxygen species at gold electrode interface and the use of electro-reduction of the produced disulfides for the electro-analysis of low levels of GSH is reported.

The thiol to disulfide inter-conversion plays vital role in the glutathione (GSH) metabolism in human body. Mimicking the GSH conversion to disulfide by electro-generated reactive oxygen species at gold electrode interface and the use of electro-reduction of the produced disulfides for the electro-analysis of low levels of GSH is reported.Figure optionsDownload as PowerPoint slide