کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
178991 459330 2014 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Interference between field excitatory postsynaptic potentials and simultaneously recorded chronoamperometric l-glutamate currents in mouse hippocampal slices
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Interference between field excitatory postsynaptic potentials and simultaneously recorded chronoamperometric l-glutamate currents in mouse hippocampal slices
چکیده انگلیسی


• Simultaneous chronoamperometric and field potential recordings were investigated.
• fEPSPs affected capacitive components of chronoamperometric currents.
• A current-sampling method was proposed to extract faradaic signals from raw currents.

Here we report on an unexpected interaction between field excitatory postsynaptic potentials (fEPSPs) and simultaneously recorded chronoamperometric l-glutamate currents based on time-resolved (200 Hz) amperometry in mouse hippocampal slices. It was found that electric stimuli and evoked fEPSPs induced an artifact on amperometric currents due to an unintentionally formed junction between the circuits of the amperometric and fEPSP measurements. The origin of the artifact was investigated using circuit simulation. To extract a faradaic signal from the observed currents, we sampled currents when the artifact decayed, i.e., 450 ms after the stimulation. The reliability of the extracted signal was examined through the manipulation of l-glutamate release probability.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 45, August 2014, Pages 1–4
نویسندگان
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