A high-performance DNA biosensor using polyhydroxylated fullerenol as 3D matrix for probe immobilization

• Fullerenol was used as a functional platform for the DNA immobilization.
• The DNA loading density was determined to be as high as 2.24 × 1013 strands cm− 2.
• High sensitivity and fast hybridization kinetics were achieved for the biosensor.

A novel electrochemical DNA biosensor has been developed using water-soluble polyhydroxylated fullerenols as 3D matrix platform for probe DNA immobilization. Owing to the multiple merits including the unique spherical 3D nanostructure, rich OH on the outside surface, and good water-solubility of fullerenol platform, the developed biosensor revealed high probe loading density (2.24 × 1013 strands cm− 2) and fast hybridization kinetics. Also, a wide linear range from 1.0 fM to 1.0 nM with a detection limit down to 0.17 fM in target DNA detection was obtained.

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