کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
180041 459369 2012 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrochemiluminescence immunosensor for the determination of ag alpha fetoprotein based on energy scavenging of quantum dots
موضوعات مرتبط
مهندسی و علوم پایه مهندسی شیمی مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
Electrochemiluminescence immunosensor for the determination of ag alpha fetoprotein based on energy scavenging of quantum dots
چکیده انگلیسی

An electrochemiluminescence (ECL) immunosensor for ultrasensitive detection of alpha fetoprotein (AFP) was fabricated using graphene-CdS quantum dots-alginate (G-CdS QDs-AL) as the immobilizing support and CdSe/ZnS QDs as the label. CdSe/ZnS QDs could effectively scavenge the ECL of G-CdS QDs-AL composite, and the quenched ECL intensity depended linearly on the logarithm for AFP concentration in the range from 0.05 to 500 fg/mL. The detection limit was 20 ag/mL. The proposed ECL immunoassay protocol for AFP detection is stable, specific, highly sensitive and promising for clinical application.


► LOD for AFP was about 20 ag/mL, 50 times better than the best previously reported.
► Black body-like QDs labeled on second antibody open a new avenue in ultrasensitive ECL bioassays for tumor markers.
► G-CdS QDs-AL composite film is promising in developing an ECL immunosensor.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 14, Issue 1, January 2012, Pages 13–16
نویسندگان
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