کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
181895 | 459412 | 2007 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Site-specific DNA cleavage of EcoRI endounclease probed by electrochemical analysis using ferrocene capped gold nanoparticles as reporter
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
مهندسی شیمی
مهندسی شیمی (عمومی)
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Site-specific DNA cleavage of EcoRI endounclease probed by electrochemical analysis using ferrocene capped gold nanoparticles as reporter Site-specific DNA cleavage of EcoRI endounclease probed by electrochemical analysis using ferrocene capped gold nanoparticles as reporter](/preview/png/181895.png)
چکیده انگلیسی
An electrochemical method was proposed to study DNA cleavage by EcoRI endonuclease using ferrocene as electrochemical indicator. Ferrocene was not covalently crosslinked to the DNA molecule but co-immobilized on the gold nanoparticles with thiolated target DNA via Au–S bond. EcoRI–DNA interaction was monitored based on the alteration of ferrocene signal before and after digestion with EcoRI. Enzymatic cleavage specificity of EcoRI was investigated, and enzymatic cleavage at DNA-modified electrode was also monitored real-time.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Electrochemistry Communications - Volume 9, Issue 5, May 2007, Pages 1086–1090
Journal: Electrochemistry Communications - Volume 9, Issue 5, May 2007, Pages 1086–1090
نویسندگان
Yan Jin, Wu Lu, Jianqiang Hu, Xin Yao, Jinghong Li,