Synchrotron infrared confocal microspectroscopic spatial resolution or a customized synchrotron/focal plane array system enhances chemical imaging of biological tissue or cells

Spectroscopy and spatially resolved chemical imaging of biological materials using an infrared microscope is greatly enhanced with confocal image plane masking to 5–6 μm with a third generation microspectrometer and illumination with a synchrotron radiation source compared to globar illuminated and array detection or singly masked system. Steps toward this instrumental achievement are illustrated with spectra and images of biological tissue sections, including single cells, brain, aorta, and grain specimens. A recent, customized synchrotron infrared microspectrometer installation enables focal plane array detection to achieve both rapid and high definition chemical imaging. Localization of the ester carbonyl population in single modified starch granules was used to provide direct comparison of the two advanced imaging capabilities.