کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1899783 1045140 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A chemometric method to identify enzymatic reactions leading to the transition from glycolytic oscillations to waves
موضوعات مرتبط
مهندسی و علوم پایه ریاضیات ریاضیات کاربردی
پیش نمایش صفحه اول مقاله
A chemometric method to identify enzymatic reactions leading to the transition from glycolytic oscillations to waves
چکیده انگلیسی
In the present work we demonstrate that FTIR-spectroscopy is a powerful tool for the time resolved and noninvasive measurement of multi-substrate/product interactions in complex metabolic networks as exemplified by the oscillating glycolysis in a yeast extract. Based on a spectral library constructed from the pure glycolytic intermediates, chemometric analysis of the complex spectra allowed us the identification of many of these intermediates. Singular value decomposition and multiple level wavelet decomposition were used to separate drifting substances from oscillating ones. This enabled us to identify slow and fast variables of glycolytic oscillations. Most importantly, we can attribute a qualitative change in the positive feedback regulation of the autocatalytic reaction to the transition from homogeneous oscillations to travelling waves. During the oscillatory phase the enzyme phosphofructokinase is mainly activated by its own product ADP, whereas the transition to waves is accompanied with a shift of the positive feedback from ADP to AMP. This indicates that the overall energetic state of the yeast extract determines the transition between spatially homogeneous oscillations and travelling waves.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Physica D: Nonlinear Phenomena - Volume 239, Issue 11, 1 June 2010, Pages 866-872
نویسندگان
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