کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1906265 1534883 2014 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dietary restriction ameliorates haematopoietic ageing independent of telomerase, whilst lack of telomerase and short telomeres exacerbates the ageing phenotype
ترجمه فارسی عنوان
محدودیت غذایی سبب کاهش پیری خونریزی مستقل از تلومراز می شود، در حالی که کمبود تلومراز و تلومرهای کوتاه باعث تشدید فنوتیپ پیری می شود
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
چکیده انگلیسی


• We studied the effects of ageing, DR, and telomerase/telomeres on bone marrow HSPCs.
• Short telomeres (Terc−/−) cause premature haematopoietic ageing.
• The absence of telomerase (Tert−/−) augments abnormal haematopoiesis in aged mice.
• Telomerase deficiency disrupts erythropoiesis in aged mice.
• Dietary restriction ameliorates haematopoietic ageing.

Ageing is associated with an overall decline in the functional capacity of tissues and stem cells, including haematopoietic stem and progenitor cells (HSPCs), as well as telomere dysfunction. Dietary restriction (DR) is a recognised anti-ageing intervention that extends lifespan and improves health in several organisms. To investigate the role of telomeres and telomerase in haematopoietic ageing, we compared the HSPC profile and clonogenic capacity of bone marrow cells from wild type with telomerase-deficient mice and the effect of DR on these parameters.Compared with young mice, aged wild type mice demonstrated a significant accumulation of HSPCs (1.3% vs 0.2%, P = 0.002) and elevated numbers of granulocyte/macrophage colony forming units (CFU-GM, 26.4 vs 17.3, P = 0.0037) consistent with myeloid “skewing” of haematopoiesis. DR was able to restrict the increase in HSPC number as well as the myeloid “skewing” in aged wild type mice. In order to analyse the influence of short telomeres on the ageing phenotype we examined mice lacking the RNA template for telomerase, TERC−/−. Telomere shortening resulted in a similar bone marrow phenotype to that seen in aged mice, with significantly increased HSPC numbers and an increased formation of all myeloid colony types but at a younger age than wild type mice. However, an additional increase in erythroid colonies (BFU-E) was also evident. Mice lacking telomerase reverse transcriptase without shortened telomeres, TERT−/−, also presented with augmented haematopoietic ageing which was ameliorated by DR, demonstrating that the effect of DR was not dependent on the presence of telomerase in HSPCs. We conclude that whilst shortened telomeres mimic some aspects of haematopoietic ageing, both shortened telomeres and the lack of telomerase produce specific phenotypes, some of which can be prevented by dietary restriction.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 58, October 2014, Pages 113–119
نویسندگان
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