کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1906584 1046300 2013 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Cytomegalovirus (CMV)-dependent and -independent changes in the aging of the human immune system: A transcriptomic analysis
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
پیش نمایش صفحه اول مقاله
Cytomegalovirus (CMV)-dependent and -independent changes in the aging of the human immune system: A transcriptomic analysis
چکیده انگلیسی

Aging is associated with a profound reduction of the immune capacity (i.e., immunosenescence), which is manifested as increased morbidity and mortality due to infectious diseases in the elderly. The association of cytomegalovirus (CMV) with several aging-associated phenomena has been extensively characterized, e.g., the accumulation of CD8 + nonproliferative, apoptosis-resistant memory cells that have lost the expression of the costimulatory molecule CD28. However, as the CMV seroprevalence is notably high in elderly individuals, the role of CMV-independent changes has been difficult to analyze. To address this question, we performed a transcriptomic analysis (Illumina Human HT12 microarray) of the peripheral blood mononuclear cells (PBMCs) in a cohort of 90-year-old individuals (CMV seronegative, n = 6; CMV seropositive, n = 140) using the PBMCs of young CMV-seronegative individuals (n = 11) as the controls. The cell type distribution (CD3, CD4, CD8, CD28 and CD14) was analyzed using FACS. The data showed that the gene expression profiles of the CMV + and CMV − nonagenarians were different compared to the CMV − controls. Compared to the CMV − controls, 667 genes showed altered expression in the CMV − nonagenarians, and 559 genes were altered in the CMV + nonagenarians. Of these, 337 genes were common. An analysis of the canonical pathways revealed that the number of affected pathways was also different (42 in CMV −, 13 in CMV +; of these, 9 were common). Taken together, these results indicate that the CMV-dependent and CMV-independent changes in the aging of the immune system are fundamentally different.


► CMV infection is known to be an important factor in the induction of immunosenescence.
► Gene expression analysis was used to distinguish the CMV dependent and independent parameters of immunosenescence.
► The data showed that these are strikingly different.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Experimental Gerontology - Volume 48, Issue 3, March 2013, Pages 305–312
نویسندگان
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