کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1906759 | 1046314 | 2012 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Effect of premature aging on murine adipose tissue Effect of premature aging on murine adipose tissue](/preview/png/1906759.png)
To evaluate the effect of aging on adipose tissue development, subcutaneous (SC) and gonadal (GON) white and peri-aortic brown adipose tissues were analyzed of 10 and 30 week old mice deficient in the clock gene Bmal1 (brain and muscle arnt like protein 1) (Bmal1−/−) and wild-type littermates (Bmal1+/+) kept on a standard fat diet. At both ages, daily food intake was significantly decreased for Bmal1−/− mice, associated with reduced hypothalamic expression of PPARα. Between 10 and 30 weeks of age, the total body weight of Bmal1+/+ mice increased significantly, but that of Bmal1−/− mice did not change. Whereas for Bmal1+/+ mice, both SC and GON fat mass increased with age, these decreased for Bmal1−/− mice. This was associated with increased adipocyte size with age for Bmal1+/+ but not for Bmal1−/− mice. Adipose tissue related angiogenesis was not affected by genotype or aging. Peri-aortic brown adipose tissue mass in 30 week old Bmal1−/− mice was significantly reduced as compared to age-matched Bmal1+/+ mice. Comparison of gene expression profiles in SC and GON adipose tissues of both genotypes revealed very marked effects of Bmal1 gene deletion in itself on PAI-1 (4- to 13-fold downregulation), whereas the associated effect of premature aging was striking for leptin (90- to 130-fold downregulation). Thus, premature aging in Bmal1−/− mice kept on normal chow was associated with reduced adiposity.
► Reduced appetite in Bmal1−/− mice is associated with reduced expression of PPARα.
► Bmal1−/− mice do not show increased white and brown fat mass with aging.
► Adipocyte and blood vessel size and density are not affected by Bmal1 deficiency.
► Bmal1 gene deletion is associated with marked downregulation of PAI-1 in white fat.
► Premature aging induces marked downregulation of leptin in adipose tissues.
Journal: Experimental Gerontology - Volume 47, Issue 3, March 2012, Pages 256–262