Leg impairments elicit graded and sex-specific demographic responses in the tephritid fruit fly Anastrepha ludens

This study was concerned with the impact of different levels of artificial impairment (leg amputations) on male and female survival and female reproduction in the Mexican fruit fly, Anastrepha ludens. We monitored the demographic responses in a total of 100 flies of each sex that were maintained individually in 4 × 4 × 10 cm cages and subject to 1-of-11 different leg amputations (plus intact control) including cohorts in which either one front, one middle or one rear leg was severed (3 cohorts total), in which two legs were severed in different front–middle–rear combinations (6 cohorts total), or in which the two middle and one additional leg were severed (2 cohorts total). The two main findings were that: (i) although the effects on mortality of impairments were sex-specific, no universal patterns emerged that applied to either sex; and (ii) reproduction occurred in all cohorts of impaired females. Moderately-impaired flies (e.g. amputation of a single middle leg) laid nearly as many eggs in their lifetime as did intact controls. However, severely impaired flies (i.e. 3 legs amputated) laid significantly fewer eggs.