Antioxidant responses and cellular adjustments to oxidative stress

• Antioxidant responses are crucial for both redox signaling and redox damage.
• Glutathione-mediated reactions and Nrf2-Keap1 pathway are key antioxidant responses.
• Redox-related post-translational modifications activate specific signaling pathways.
• Redox-sensitive microRNAs contribute to redox-mediated gene expression regulation.
• ER stress and ischemia-reperfusion are antioxidant-related pathophysiological events.

Redox biological reactions are now accepted to bear the Janus faceted feature of promoting both physiological signaling responses and pathophysiological cues. Endogenous antioxidant molecules participate in both scenarios. This review focuses on the role of crucial cellular nucleophiles, such as glutathione, and their capacity to interact with oxidants and to establish networks with other critical enzymes such as peroxiredoxins. We discuss the importance of the Nrf2-Keap1 pathway as an example of a transcriptional antioxidant response and we summarize transcriptional routes related to redox activation. As examples of pathophysiological cellular and tissular settings where antioxidant responses are major players we highlight endoplasmic reticulum stress and ischemia reperfusion. Topologically confined redox-mediated post-translational modifications of thiols are considered important molecular mechanisms mediating many antioxidant responses, whereas redox-sensitive microRNAs have emerged as key players in the posttranscriptional regulation of redox-mediated gene expression. Understanding such mechanisms may provide the basis for antioxidant-based therapeutic interventions in redox-related diseases.

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