کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1923043 1535848 2014 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High throughput assay for evaluation of reactive carbonyl scavenging capacity
ترجمه فارسی عنوان
تست سرعت بالا برای ارزیابی ظرفیت تخلیه کربنیک واکنشی؟
کلمات کلیدی
گونه های کربونی بازدارنده، کربنیل خواص، آلودگی فلورسنت، تست غربالگری
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی سالمندی
چکیده انگلیسی


• We describe a rapid method for assessment of reactive carbonyl scavengers.
• We evaluated the carbonyl scavenger activity of various pharmacophores.
• α-amino-β-mercaptoethane structure showed the highest degree of activity.

Many carbonyl species from either lipid peroxidation or glycoxidation are extremely reactive and can disrupt the function of proteins and enzymes. 4-hydroxynonenal and methylglyoxal are the most abundant and toxic lipid-derived reactive carbonyl species. The presence of these toxics leads to carbonyl stress and cause a significant amount of macromolecular damages in several diseases. Much evidence indicates trapping of reactive carbonyl intermediates may be a useful strategy for inhibiting or decreasing carbonyl stress-associated pathologies. There is no rapid and convenient analytical method available for the assessment of direct carbonyl scavenging capacity, and a very limited number of carbonyl scavengers have been identified to date, their therapeutic potential being highlighted only recently. In this context, we have developed a new and rapid sensitive fluorimetric method for the assessment of reactive carbonyl scavengers without involvement glycoxidation systems. Efficacy of various thiol- and non-thiol-carbonyl scavenger pharmacophores was tested both using this screening assay adapted to 96-well microplates and in cultured cells. The scavenging effects on the formation of Advanced Glycation End-product of Bovine Serum Albumin formed with methylglyoxal, 4-hydroxynonenal and glucose-glycated as molecular models were also examined. Low molecular mass thiols with an α-amino-β-mercaptoethane structure showed the highest degree of inhibitory activity toward both α,β-unsaturated aldehydes and dicarbonyls. Cysteine and cysteamine have the best scavenging ability toward methylglyoxal. WR-1065 which is currently approved for clinical use as a protective agent against radiation and renal toxicity was identified as the best inhibitor of 4-hydroxynonenal.

Reaction of carbonyl compounds with NBD-H to form highly fluorescent derivatives. Fluorescence is strongly inhibited by carbonyl scavengers such as thiols with an α-amino-β-mercaptoethane structure.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Redox Biology - Volume 2, 2014, Pages 590–598
نویسندگان
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