کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1923371 | 1048889 | 2016 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Acetylation of histones in neocortex and hippocampus of rats exposed to different modes of hypobaric hypoxia: Implications for brain hypoxic injury and tolerance
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کلمات کلیدی
HDACsDNMTsDNA methyltransferasesSevere hypoxiaHypoxic preconditioning - preconditioning hyppoxicHistone acetylation - استیلیت هیستونhistone deacetylases - هیستون deacetylasesHistone H3 - هیستون H3histone acetyltransferases - هیستون استیل ترانسفرازHypoxia - هیپوکسیHATs - کلاهMineralocorticoid receptor - گیرنده مینرالوکورتیکوئید glucocorticoid receptor - گیرنده گلوکوکورتیکوئید
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Acetylation of histones in neocortex and hippocampus of rats exposed to different modes of hypobaric hypoxia: Implications for brain hypoxic injury and tolerance Acetylation of histones in neocortex and hippocampus of rats exposed to different modes of hypobaric hypoxia: Implications for brain hypoxic injury and tolerance](/preview/png/1923371.png)
چکیده انگلیسی
Acetylation of nucleosome histones results in relaxation of DNA and its availability for the transcriptional regulators, and is generally associated with the enhancement of gene expression. Although it is well known that activation of a variety of pro-adaptive genes represents a key event in the development of brain hypoxic/ischemic tolerance, the role of epigenetic mechanisms, in particular histone acetylation, in this process is still unexplored. The aim of the present study was to investigate changes in acetylation of histones in vulnerable brain neurons using original well-standardized model of hypobaric hypoxia and preconditioning-induced tolerance of the brain. Using quantitative immunohistochemistry and Western blot, effects of severe injurious hypobaric hypoxia (SH, 180Â mm Hg, 3Â h) and neuroprotective preconditioning mode (three episodes of 360Â mm Hg for 2Â h spaced at 24Â h) on the levels of the acetylated proteins and acetylated H3 Lys24 (H3K24ac) in the neocortex and hippocampus of rats were studied. SH caused global repression of the acetylation processes in the neocortex (layers II-III, V) and hippocampus (CA1, CA3) by 3-24Â h, and this effect was prevented by the preconditioning. Moreover, hypoxic preconditioning remarkably increased the acetylation of H3K24 in response to SH in the brain areas examined. The preconditioning hypoxia without subsequent SH also stimulated acetylation processes in the neocortex and hippocampus. The moderately enhanced expression of the acetylated proteins in the preconditioned rats was maintained for 24Â h, whereas acetylation of H3K24 was intense but transient, peaked at 3Â h. The novel data obtained in the present study indicate that large activation of the acetylation processes, in particular acetylation of histones might be essential for the development of brain hypoxic tolerance.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Acta Histochemica - Volume 118, Issue 2, March 2016, Pages 80-89
Journal: Acta Histochemica - Volume 118, Issue 2, March 2016, Pages 80-89
نویسندگان
Mikhail Samoilov, Anna Churilova, Tatjana Gluschenko, Oleg Vetrovoy, Natalia Dyuzhikova, Elena Rybnikova,