کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1977412 1061487 2012 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Factors influencing the assimilation of arsenic in a deposit-feeding polychaete
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Factors influencing the assimilation of arsenic in a deposit-feeding polychaete
چکیده انگلیسی

We investigated mechanisms leading to the assimilation of particle-bound arsenic (As) ingested by the deposit-feeding polychaete Alitta succinea using a radiotracer approach.The release of As from different particle types into extracted gut fluid or bovine serum albumin (BSA), a gut fluid mimic, was measured. In addition, gut fluid proteins were analyzed by separating proteins via 2D gel electrophoresis, and protein peptide sequences were determined by mass spectrometry. Major ions in the gut fluid were measured by ion chromatography and metals by mass spectrometry. Percentages of particulate As release were related to As assimilation efficiencies (AEs) in polychaetes feeding on different particle types. AEs of As were highest from radiolabeled pure diatoms (72%) and radiolabeled diatoms added to sediment (51%), lower from radiolabeled sediment (10%), and lowest from a radiolabeled iron oxide mineral, goethite (2%). It appears that As release from particles is a necessary but not sufficient requirement of As assimilation. For example, 15% of As was released from goethite into the gut fluid but only 2% was assimilated by A. succinea. Our results suggest that the likelihood of As assimilation is higher when it is bound to an organic compound of nutritional value in the ingested particles.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 156, Issue 1, June 2012, Pages 42–50
نویسندگان
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