کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1977891 | 1061516 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: The characterization of cytosolic glutathione transferase from four species of sea turtles: Loggerhead (Caretta caretta), green (Chelonia mydas), olive ridley (Lepidochelys olivacea), and hawksbill (Eretmochelys imbricata) The characterization of cytosolic glutathione transferase from four species of sea turtles: Loggerhead (Caretta caretta), green (Chelonia mydas), olive ridley (Lepidochelys olivacea), and hawksbill (Eretmochelys imbricata)](/preview/png/1977891.png)
Glutathione s-transferases (GST) play a critical role in the detoxification of exogenous and endogenous electrophiles, as well as the products of oxidative stress. As compared to mammals, GST activity has not been extensively characterized in reptiles. Throughout the globe, most sea turtle populations face the risk of extinction. Of the natural and anthropogenic threats to sea turtles, the effects of environmental chemicals and related biochemical mechanisms, such as GST catalyzed detoxification, are probably the least understood. In the present study, GST activity was characterized in four species of sea turtles with varied life histories and feeding strategies: loggerhead (Caretta caretta), green (Chelonia mydas), olive ridley (Lepidochelys olivacea), and hawksbill (Eretmochelys imbricata). Although similar GST kinetics was observed between species, rates of catalytic activities using class-specific substrates show inter- and intra-species variation. GST from the spongivorous hawksbill sea turtle shows 3–4.5 fold higher activity with the substrate 4-nitrobenzylchloride than the other 3 species. GST from the herbivorous green sea turtle shows 3 fold higher activity with the substrate ethacrynic acid than the carnivorous olive ridley sea turtle. The results of this study may provide insight into differences in biotransformation potential in the four species of sea turtles and the possible health impacts of contaminant biotransformation by sea turtles.
Journal: Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology - Volume 150, Issue 2, August 2009, Pages 279–284