Hepatoprotective and proteomic mechanism of Sphaeranthus indicus in paracetamol induced hepatotoxicity in wistar rats

• Hepatoprotective and proteomic analysis on Sphaeranthus indicus extract using rats.
• Sustain the antioxidant status.
• Sphaeranthus indicus could be used as food supplement.

The present study was carried out to explore the hepatoprotective and proteomic mechanisms of Sphaeranthus indicus in paracetamol induced hepatotoxicity in rats using 2D gel and MALDI-TOF-MS. Oral administration of ethanolic and aqueous extract of S. indicus to the paracetamol induced rats' showed increase of both enzymatic and non-enzymatic antioxidant status and decreased lipid peroxide level. For proteomic approach, identified the four differential spots were expressed in paracetamol induced and S. indicus treated rats through 2D gel. The expressions of these protein spots were further confirmed by MALDI-TOF-MS i.e. Heat shock protein, Liver transferrin, Glutathione-S-Transferase and Carnitine palmitoyl transferase. These identified proteins involved in many cellular activities like maintain cellular integrity, iron transport, free radical scavenging activity and β oxidation of fatty acids. These results were given more information about antioxidant activity of extracts. Therefore, the plant extracts could be used safely as food supplement for antioxidant activities enhancements.