کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1985543 1540227 2016 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Dromedary immune response and specific Kv2.1 antibody generation using a specific immunization approach
ترجمه فارسی عنوان
پاسخ ایمنی Dromedary و تولید آنتی بادی ویژه Kv2.1 با استفاده از روش ایمن سازی خاص
کلمات کلیدی
سرم اختصاصی Kv؛ پاسخ ایمنی؛ سلول ELISA؛ گیره پچ
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی

Voltage-gated potassium (Kv) channels form cells repolarizing power and are commonly expressed in excitable cells. In non-excitable cells, Kv channels such as Kv2.1 are involved in cell differentiation and growth. Due to the involvement of Kv2.1 in several physiological processes, these channels are promising therapeutic targets. To develop Kv2.1 specific antibody-based channel modulators, we applied a novel approach and immunized a dromedary with heterologous Ltk- cells that overexpress the mouse Kv2.1 channel instead of immunizing with channel protein fragments. The advantage of this approach is that the channel is presented in its native tetrameric configuration. Using a Cell-ELISA, we demonstrated the ability of the immune serum to detect Kv2.1 channels on the surface of cells that express the channel. Then, using a Patch Clamp electrophysiology assay we explored the capability of the dromedary serum in modulating Kv2.1 currents. Cells that were incubated for 3 h with serum taken at Day 51 from the start of the immunization displayed a statistically significant 2-fold reduction in current density compared to control conditions as well as cells incubated with serum from Day 0. Here we show that an immunization approach with cells overexpressing the Kv2.1 channel yields immune serum with Kv2.1 specific antibodies.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: International Journal of Biological Macromolecules - Volume 93, Part A, December 2016, Pages 167–171
نویسندگان
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