کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1988670 1540444 2016 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Validation of antibodies for neuroanatomical localization of the P2Y11 receptor in macaque brain
ترجمه فارسی عنوان
اعتبار سنجی آنتی بادی برای محلی سازی neuroanatomical گیرنده P2Y11 در مغز نوعی میمون دنیای قدیم
کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
چکیده انگلیسی


• Two human P2Y11 receptor antibodies are suitable for western blot and flow cytometry.
• The two P2Y11 antibodies had a similar staining pattern in rat and macaque brain.
• The two P2Y11 antibodies were not considered specific in immunohistochemistry of macaque brain.

Focus on the purinergic receptor P2Y11 has increased following the finding of an association between the sleep disorder narcolepsy and a genetic variant in P2RY11 causing decreased gene expression. Narcolepsy is believed to arise from an autoimmune destruction of the hypothalamic neurons that produce the neuropeptide hypocretin/orexin. It is unknown how a decrease in expression of P2Y11 might contribute to an autoimmune reaction towards the hypocretin neurons and the development of narcolepsy. To advance narcolepsy research it is therefore extremely important to determine the neuroanatomical localization of P2Y11 in the brain with particular emphasis on the hypocretin neurons. In this article we used western blot, staining of blood smears, and flow cytometry to select two antibodies for immunohistochemical staining of macaque monkey brain. Staining was seen in neuron-like structures in cortical and hypothalamic regions. Rats do not have a gene orthologue to the P2Y11 receptor and therefore rat brain was used as negative control tissue. The chromogenic signal observed in macaque monkey brain in neurons was not considered reliable, because the antibodies stained rat brain in a similar distribution pattern. Hence, the neuroanatomical localization of the P2Y11 receptor remains undetermined due to the lack of specific P2Y11 antibodies for brain immunohistochemistry.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chemical Neuroanatomy - Volume 78, December 2016, Pages 25–33
نویسندگان
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