کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2008025 | 1066393 | 2007 | 9 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Copper-binding activity of Trefoil factor 1 (TFF1): A new perspective in the study of the multifunctional roles of TFFs
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
ESI-MSIMACTFF1LC–MS - LC-MSliquid chromatography–mass spectrometry - طیف سنجی جرم کروماتوگرافی مایعElectrospray ionization-mass spectrometry - طیف سنجی جرم یونیزه الکترو اسپریtrefoil factor - عامل تراکمSELDI-TOF - فروخته شده-TOFCopper-binding protein - پروتئین متصل به مسimmobilized metal affinity chromatography - کروماتوگرافی وابسته به فلز متمرکز
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Trefoil factors (TFFs) are gastrointestinal peptides playing an essential role in the epithelial restitution. Among the three known TFF peptides, TFF1 is characterized by three disulfide bonds producing a compact globular structure and an extended and disordered tail formed by amino- and carboxy-termini. The presence of a cysteine surrounded by several negatively charged residues in this region of the protein, highly conserved in different species, suggests the possible formation of a metal-binding site. Affinity chromatography and mass spectrometric analyses allowed us to demonstrate a selective binding affinity of TFF1 for copper. The binding induces conformational changes in the tertiary structure as demonstrated by circular dichroism experiments, while limited proteolysis revealed an altered access to the cleavage sites in the amino- and carboxy-termini. The results of this study reveal a new property of TFF1 and suggest that copper could influence its biological activities by interfering with the dimerization of the peptide and/or the interaction with mucins or putative TFF receptors.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 28, Issue 7, July 2007, Pages 1461-1469
Journal: Peptides - Volume 28, Issue 7, July 2007, Pages 1461-1469
نویسندگان
Alessandra Tosco, Maria Chiara Monti, Bianca Fontanella, Marie-Christine Rio, Luigi Gomez-Paloma, Arturo Leone, Liberato Marzullo,