Keywords: Zinc finger; Protein purification; Immobilized metal affinity chromatography; IMAC;
مقالات ISI (ترجمه نشده)
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Keywords: Chitosan-NTA coating; Nanocomposites; Protein purification; AB-NTA; N-(5-Amino-1-carboxy-pentyl) iminodiacetic acid; TEM; Transmission electron microscopy; SEM; scanning electron microscopy; FTIR; Fourier transform infrared; YesZ; β-galactosidase from Ba
Keywords: WNV; West Nile virus; EDIII; envelope protein domain III; C; capsid; E; envelope; NS; nonstructural; IB; inclusion bodies; IMAC; immobilized metal affinity chromatography; IEX; ion exchange; LB; Luria Bertani; TB; terrific broth; IPTG; isopropyl β-d-1-th
Keywords: Sample pretreatment; enrichment; nanomaterial; low-abundance proteins/peptides; phosphoproteins/phosphopetides; glycoproteins/glycopeptides; AAPBA; poly 3-acrylaminophenylboronic acid; AEX; anion-exchange chromatography; AFP; α-fetoprotein; AgNPs; silver
Keywords: EDTA; ethylenediamine tetraacetic acid; HA; hyaluronic acid; HMW; high molecular weight; IMAC; immobilised metal affinity chromatography; IPTG; isopropyl β-d-1-thiogalactopyranoside; LMW; low molecular weight; MALDI-MS; matrix-assisted laser desorption i
Keywords: AcMNPV; Autographa californica nuclearpolyhedrovirus; BAC; bacterial artificial chromosome; BEVS; baculovirus expression vector system; DNA; deoxyribonucleic acid; ds; double stranded; E. coli; Escherichia coli; YFP; yellow fluorescent protein; kDa; kilo
Keywords: CD; circular dichroism (spectroscopy); CI; compaction index; κCR; κ-normalized compaction responsiveness; DLS; dynamic light scattering; ESRF; European Synchrotron Radiation Facility; FCR; fraction of charged residues; HeV; Hendra virus; IDP/IDR; intrin
Keywords: Mitochondria; Proteomics; Mass spectrometry; Cancer; 1DE; one-dimensional gel electrophoresis; 2DE; two-dimensional gel electrophoresis; 2D-DIGE; fluorescent two-dimensional difference gel electrophoresis; AP; affinity purification; ACAT1; mitochondrial a
Keywords: Fusion protein; Expression; Solubility; Purification; Solubility-enhancing tag; Affinity tag; R9AP; RGS9-1 anchor protein; CMC; critical micellar concentration; GST; glutathione S-transferase; IMAC; immobilized metal affinity chromatography; IPTG; 1-Propy
Targeted mass spectrometry: An emerging powerful approach to unblock the bottleneck in phosphoproteomics
Keywords: DDA; data dependent acquisition; IMAC; immobilized metal affinity chromatography; IP; immunoprecipitation; MS; mass spectrometry; pS; phosphoserine; pT; phosphothreonine; pY; phosphotyrosine; PPSA; phospho-specific antibody; PRM; parallel reaction monitor
Keywords: AA; antimycin A; CSA; cyclosporin A; ETC; Electron transport chain; IMAC; Inner membrane anion channel; OXPHOS; Oxidative phosphorylation; Mitochondrial calcium uniporter; Mitochondrial sodium-calcium exchanger; Whole-mitoplast electrophysiology; Cardiac
Keywords: CID; Collision-induced dissociation; CPP; Calcium phosphate precipitation; ETD; Electron transfer dissociation; HILIC; Hydrophilic interaction chromatography; IMAC; Immobilized metal affinity chromatography; iTRAQ; Isobaric tags for relative and absolute
Recent advances and applications of polydopamine-derived adsorbents for sample pretreatment
Keywords: Polydopamine (PDA); Sorption-based extraction; Adsorbents; Sample pretreatment; Solid-phase extraction (SPE); Solid-phase microextraction (SPME); Stir bar sorptive extraction (SBSE); Magnetic solid-phase extraction (MSPE); AA; acrylamide; BMOs; binary met
Keywords: BCA; bicinchoninic acid; CDR1; 2complementarity determining regions 1, 2; CFA; complete Freund's adjuvant; Cκ; constant kappa light chain; Cλ; constant lambda light chain; DH; heavy diversity; DNase; deoxyribonuclease; ELISA; direct enzyme-linked immu
Keywords: AtSerpin1; Arabidopsis thaliana serpin 1; BSA; bovine serum albumin; Bt; Bacillus thuringiensis; IMAC; immobilized metal-affinity chromatography; LR serpin; serpin with leucine and arginine at positions P2 and P1 of the reactive center loop; RCL; reactive
Keywords: FTICR MS; Fourier transform ion cyclotron mass spectrometry; MALDI; matrix-assisted laser desorption ionization; IMAC; immobilized metal affinity chromatography; CID; collision induced dissociation; DTT; dl-Dithiothreitol; DSP; average degree of substitut
Keywords: Microcystin; Pumice; Cryogels; IMAC; Water treatment;
Keywords: Dengue virus; Envelope protein domain III; Lipoprotein; Vaccine; D3ED III; ecombinant dengue-3 envelope protein domain III; ED III/Alum; D3ED III formulated with aluminum phosphate; FFU; focus-forming unit; FRNT; focus reduction neutralization tests; IMAC
Keywords: 2D-LC; two-dimensional liquid chromatography; AA; amino acid; AC; affinity chromatography; ADC; antibody-drug conjugates; ADCC; antibody-dependent cellular cytotoxicity; AF4; asymmetric flow field fractionation; APTS; 1-aminopyrene-3,6,8-trisulfonate; Asn
Keywords: AKH; adipokinetic hormone; CHO; Chinese hamster ovary; Crypα; cell adhesion molecule-like tyrosine phosphatase α; Endo H; Endoglycosidase H; ENG; endoglin/CD105; HEK; human embryonic kidney; IMAC; immobilized metal affinity chromatography; MBP; maltose-
Keywords: Îp; proton motive force established on inner mitochondrial membrane; ÎÏ; electrical membrane potential established on inner mitochondrial membrane; COX4.1; cytochrome c oxidase subunit 4 isoform 1; ERK; extracellular signal-regulated kinase; FA; fatty
Keywords: BFRF3; BamHI F rightward reading frame 3; IBs; inclusion bodies; IMAC; immobilized metal ion affinity chromatography; IPTG; isopropyl-β-d-thiogalactopyranoside; IV; index value; NPC; nasopharyngeal carcinoma; PBST; phosphate-buffered saline-Tween-20; PP;
Keywords: Collagen; Chelating/binding peptides; IMAC; Functional food; ESI-MS;
Keywords: Ac2; EpCAM-binding DARPin; ATCC; American Type Culture Collection; Avi tag; peptide tag that is biotinylated by BirA; BirA; E. coli biotin ligase; CPP; cell-penetrating peptide; DARPin; designed ankyrin repeat protein; Ec1; EpCAM-binding DARPin; EpCAM; ep
Keywords: AEX; anion exchange chromatography; CID; collision induced dissociation; DHB; 2,5-dihydroxybenzoic acid; ECD; electron capture dissociation; EGF; epidermal growth factor; EGFR; epidermal growth factor receptor; EGFRvIII; epidermal growth factor receptor v
Keywords: Controlled immobilization; Enzyme stabilization; Multimeric enzymes; Chimeric proteins; Covalent attachment; Ionic exchange; IMAC;
Keywords: Variable domain of heavy-chain antibody; Inhibition of protein misfolding and aggregation; Protein misfolding diseases; Amyloidoses; VHH; Nanobody; Ab; antibody; Aβ; amyloid β-peptide; AD; Alzheimer's disease; ANS; anilino naphthalene-sulfonic acid; AP;
Keywords: MCP; metal-chelating peptides; IMAC; immobilized metal affinity chromatography; RP-HPLC; reversed-phase high-performance liquid chromatography; ESI-MS; electrospray ionization mass spectrometry; RPH; rapeseed protein hydrolysates; ECT; EDTA complexing tit
Keywords: Cellular prion protein; SEC; IMAC; Purification; Western blot
Keywords: Proteomics; Affinity chromatography; Phosphopeptides; SIMAC; IMAC; MOAC
Keywords: CID; collision induced dissociation; ECD; electron capture dissociation; ETD; electron transfer dissociation; ETcaD; ETD with (supplemental activation); FDR; false discovery rate; FLR; false localisation rate; IMAC; immobilised metal affinity chromatograp
Keywords: HHP; high hydrostatic pressure; GSSG and GSH; oxidized and reduced form of glutathione* respectively; IB; inclusion bodies; CD; circular dichroism; IMAC; immobilized metal affinity chromatography; LS; light scattering; GdnHCl; guanidine hydrochloride; Pro
Keywords: Termite; Glycoside hydrolase; Molecular docking; Coptotermes gestroi; Synergistic interaction; APTS; 8-aminopyreno-1,3,6-trisulfonic acid; BIN; integral sugarcane bagasse; CD; circular dichroism; CMC; carboxymethyl cellulose; CZE; capillary zone electroph
Keywords: IMP; integral membrane protein; GFP; green fluorescent protein; FSEC; fluorescence-detected size-exclusion chromatography; IMAC; immobilized metal affinity chromatography purification; SEC; size-exclusion chromatography; GF; gel filtration; AGF; analytica
Keywords: 2xYT; double strength YT medium; AUC; analytical ultracentrifugation; CHAPS; 3-[(3-cholamidopyropyl)dimethylammonio]-1-propanesulfonate; CHS; cholesteryl hemisuccinate Tris salt; DDM; n-dodecyl-β-d-maltoside; DLS; dynamic light scattering; E. coli; Esche
Expression, purification, and characterisation of human soluble Epoxide Hydrolase (hsEH) and of its functional C-terminal domain
Keywords: Soluble epoxide hydrolase; Escherichia coli; Human embryonic kidney 293 free cells; Recombinant expression; C-terminal domain; EETs; epoxieicosatrienoic acids; AR9281; 1-(1-acetylpiperidin-4-yl)-3-adamantan-1-ylurea; AUDA; 12-[[(tricyclo[3.3.1.13,7]dec-1-
Structural requirement of the hydrophobic region of the Bordetella pertussis CyaA-hemolysin for functional association with CyaC-acyltransferase in toxin acylation
Keywords: CyaA-RTX; CyaC-acyltransferase; Electrostatic potentials; Hydrophobic region; Palmitoylation; Protein association; AR; acylation region; CyaA; adenylate cyclase-hemolysin toxin; IMAC; immobilized-metal ion affinity chromatography; HP; hydrophobic region;
Purification and characterization of the antibacterial peptidase lysostaphin from Staphylococcus simulans: Adverse influence of Zn2+ on bacteriolytic activity
Keywords: Bacteriocin lysostaphin; Inhibitory metal-binding site; Staphylolytic activity; Zn2+ chelation; Zn2+-dependent endopeptidase; IMAC; immobilized metal ion affinity chromatography; MRSA; methicillin-resistant Staphylococcus aureus strain; Lss; the â¼28-kDa
Bioanalysis of ibrutinib, and its dihydrodiol- and glutathione cycle metabolites by liquid chromatography-tandem mass spectrometry
Keywords: BTK; Bruton's tyrosine kinase; CGS; cysteinylglycine dipeptide; CYS; cysteine (amino acid); DHI; dihydrodiol-ibrutinib; DHP-I; dihydropeptidase-I; DMSO; dimethyl sulfoxide; GLU; glutamate (amino acid); GSH; glutathione tripeptide; GST; glutathione-S-trans
Humanin directly protects cardiac mitochondria against dysfunction initiated by oxidative stress by decreasing complex I activity
Keywords: HNG; Humanin analogue; CDP; 4â²-chlorodiazepam; IMAC; inner membrane anion channel; CsA; cyclosporine A; mPTP; mitochondrial permeability transition pore; ROS; reactive oxygen species; Humanin; Heart; Oxidative stress; Mitochondrial dysfunction; Ischemia
Systematic optimization of cell-free synthesized human endothelin B receptor folding
Keywords: RT; room temperature; SEC; size exclusion chromatography; IMAC; immobilized metal-ion affinity chromatography; CV; column volume; CE; continuous exchange; CF; cell-free; RM; reaction mixture; FM; feeding mixture; DTT; dithiothreitol; TCEP; tris-2-carboxye
The parasite-derived rOv-ASP-1 is an effective antigen-sparing CD4+ T cell-dependent adjuvant for the trivalent inactivated influenza vaccine, and functions in the absence of MyD88 pathway
Keywords: HA; Hemagglutinin; IMAC; Immobilized Metal Affinity Chromatography; IPTG; Isopropyl β-D-1-thiogalactopyranoside; rOv-ASP-1; Recombinant Onchocerca volvulus activation associated protein-1; Th cells; T helper cells; T-PER; Tissue protein extraction reagen
O-carboxymethyl chitosan Schiff base complexes as affinity ligands for immobilized metal-ion affinity chromatography of lysozyme
Keywords: Cryogel; IMAC; Carboxymethyl chitosan; Lysozyme adsorption;
ROS-induced ROS release in plant and animal cells
Keywords: IMAC; Inner mitochondrial membrane anion channel; mPTP; Mitochondrial permeability transition pore; NOX; NADPH oxidase; RBOH; respiratory burst oxidase homolog; RIRR; ROS-induced ROS release; ROS; reactive oxygen species; VDAC; Voltage-dependent anion cha
Biophysical characterization and stabilization of detergent-solubilized lipoprotein N-acyl transferase from P. aeruginosa and E. coli
Keywords: ε; molar extinction coefficient (Mâ1â¯cmâ1); EDTA; ethylenediaminetetraacetic acid; HEPES; 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid; HRP; horseradish peroxidase; IMAC; immobilized-metal affinity chromatography; IPTG; isopropyl β-d-1-thiog
Selective release of overexpressed recombinant proteins from E. coli cells facilitates one-step chromatographic purification of peptide-tagged green fluorescent protein variants
Keywords: Selective release; Selective liberation; One-step; Freeze-thaw; Protein purification; Green fluorescent protein GFP; %B; percentage of elution buffer; AEC; anion exchange chromatography; BCA; bicinchoninic acid; CV; column volume; GFP; green fluorescent p
Production of authentic geranylgeranylated KRAS4b using an engineered baculovirus system
Keywords: Prenylation; RAS; Geranylgeranylation; Protein production; Baculovirus; FNT; farnesyltransferase; GGTase; geranylgeranyltransferase; MBP; maltose-binding protein; FMe; farnesylated and methylated; GGMe; geranylgeranylated and methylated; GG; geranylgerany
Evaluation of in vitro refolding vs cold shock expression: Production of a low yielding single chain variable fragment
Keywords: Inclusion bodies; Single chain variable fragment; In vitro protein refolding; Cold shock; Disulfide bridge; Tissue factor; Fusion partner; Codon Adaptation Index; CAI; Dimethyl sulfoxide; DMSO; E.coli Codon Usage Analyzer; ECUA; Escherichia coli; E.coli;
Optimized expression of the Starmerella bombicola lactone esterase in Pichia pastoris through temperature adaptation, codon-optimization and co-expression with HAC1
Keywords: Lipase; Lactonase; Starmerella bombicola; Pichia pastoris; Protein purification; Green chemistry; rSBLE; Recombinant Starmerella bombicola lactone esterase; rSBLEopt; Recombinant Starmerella bombicola lactone esterase obtained after codon-optimization; En
Secretion, isotopic labeling and deglycosylation of N-acylethanolamine acid amidase for biophysical studies
Keywords: Cysteine-hydrolase; Isotopic-labeling; Purification; Ion-exchange; IMAC; Deglycosylation; MALDI-TOF; NMR;