Super induction of dengue virus NS1 protein in E. coli
Keywords: Dengue; NS1; E. coli expression; IMAC; Refolding
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Immobilized metal affinity chromatography of histidine-tagged lentiviral vectors using monolithic adsorbents
Keywords: Monolith; Cryogel; IMAC; Lentiviral vector; Gene therapy; Purification
Automated sample preparation method for mass spectrometry analysis on recombinant proteins
Keywords: Mass spectrometry; High-throughput; Automated sample preparation; Recombinant proteins; IMAC; RPLC;
Myrosinases from root and leaves of Arabidopsis thaliana have different catalytic properties
Keywords: IMAC; immobilised metal affinity chromatography; pNPG; p-nitrophenyl β-d-glucopyranoside; TGG; thioglucoside glucosidase; Arabidopsis thaliana; Brassicaceae; Glucosidase; Glucosinolate; Myrosinase;
First evidence for the salt-dependent folding and activity of an esterase from the halophilic archaea Haloarcula marismortui
Keywords: Af EST; Archeoglobus fulgidus esterase; CD; circular dichroism; DMSO; dimethyl sulfoxide; E600; diethyl-p-nitrophenyl phosphate; EST2; Alicyclobacillus acidocaldarius esterase; HCA; hydrophobic cluster analysis; Hm EST; esterase from Haloarcula marismortu
Identification of uranyl binding proteins from human kidney-2 cell extracts by immobilized uranyl affinity chromatography and mass spectrometry
Keywords: IMAC; Protein–metal interactions; Uranium; Proteomics; Metal-docking prediction algorithm; Aminophosphonate groups
Novel channels of the inner mitochondrial membrane
Keywords: ANT; Adenine Nucleotide Translocator; BHT; 3,5-di-t-Butyl-4-HydroxyToluene; BKCa; Big Ca-dependent K+ channel; CaM; Calmodulin; CLIC; Chloride-selective Intracellular Channel; CSA; Cyclosporin A; diCl-DHAA; 12,14-dichlorodehydroabietic acid; ÎÏm; mitoch
Proteomic profiling of phosphoproteins and glycoproteins responsive to wild-type alpha-synuclein accumulation and aggregation
Keywords: ACN; acetonitrile; α-Syn; wild-type alpha-synuclein; AD; Alzheimer's disease; BCA; bicinchoninic acid assay; 2-D; two-dimensional; FA; formic acid; IEF; isoelectric focusing; IMAC; immobilized metal ion affinity chromatography; PAGE; polyacrylamide gel e
Purification and analysis of polyhistidine-tagged human parvovirus B19 VP1 and VP2 expressed in insect cells
Keywords: Baculovirus; Human parvovirus B19; VLPs; IMAC; Polyhistidine tag
A new metal-chelated beads for reversible use in uricase adsorption
Keywords: Uricase; Enzyme immobilization; IMAC
Identification of serine phosphorylation in mitochondrial uncoupling protein 1
Keywords: ACN; acetonitrile; BAT; brown adipose tissue; BSA; bovine serum albumin; FBS; foetal bovine serum; HTP; hydroxyapatite; IMAC; Immobilized Metal Affinity Chromatography; Octyl-POE; octylpentaoctylethylene ether; SDS-PAGE; sodium dodecylsulphate polyacrylam
A novel role for thioredoxin reductase in the iron metabolism of S. cerevisiae
Keywords: Iron; 2-DE; IMAC; Saccharomyces cerevisiae; Thioredoxin reductase
Sequence-specific binding of DNA and RNA to immobilized Nickel ions
Keywords: Nickel; IMAC; Aptamer; Adenine; In vitro selection; Paramagnetic beads
Biochemical and biophysical properties of a highly active recombinant arginase from Leishmania (Leishmania) amazonensis and subcellular localization of native enzyme
Keywords: NO; nitric oxide; iNOS; inducible nitric oxide synthase; GFP; green fluorescent protein; ORF; open reading frame; IMAC; immobilized metal affinity chromatography; IEC; ionic exchange chromatography; IFA; immunofluorescent antibody analysis; Protein struct
A poly(2-hydroxyethyl methacrylate)-based immobilized metal affinity chromatography adsorbent for protein purification
Keywords: poly(HEMA); d-Hydantoinase; IMAC; Protein purification
Use of ProteinChip technology for identifying biomarkers of parasitic diseases: The example of porcine cysticercosis (Taenia solium)
Keywords: Pig; Taenia solium; Cysticercosis; Cestode; Biological markers; Surface Enhanced Laser Desorption-Ionization Time of Flight (SELDI-TOF); Apo A-I; apolipoprotein A-I; Apo A-IV; apolipoprotein A-IV; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-1-propanes
Adsorption behaviors of recombinant proteins on hydroxyapatite-based immobilized metal affinity chromatographic adsorbents
Keywords: IMAC; Adsorption isotherm; Protein purification
Effect of IDA and TREN chelating agents and buffer systems on the purification of human IgG with immobilized nickel affinity membranes
Keywords: Affinity membrane; Purification; Human IgG; Adsorption; IMAC
Cyclododecane and cyclotridecane complexes with cobalt as potent histidine chelators: A theoretical study
Keywords: Cyclotridecanes; Cyclododecanes; IMAC; DFT;
Glycan chains modulate prion protein binding to immobilized metal ions
Keywords: Prion; Copper; PrP glycoforms; IMAC; Scrapie
Expression of human BK ion channels in Sf9 cells, their purification using metal affinity chromatography, and functional reconstitution into planar lipid bilayers
Keywords: IMAC; His-tag; Ion channel; Sf9 insect cell; Purification; Planar lipid bilayer
One-trial in vitro conditioning regulates an association between the β-thymosin repeat protein Csp24 and actin
Keywords: actin-binding; excitability; protein kinase C; phosphorylation; beta-thymosin repeat protein; ASW; artificial sea water; CS; conditioned stimulus; Csp24; 24 kDa conditioned stimulus pathway phosphoprotein (accession number AAN08024); CV; column volume; GF
Identification and quantification of histidine-rich glycoprotein (HRG) in the blood plasma of six marine bivalves
Keywords: Bivalve; Blood plasma; Cadmium; ELISA; HRG; IMAC; Metal carrier; Transport protein
Current methods for phosphoprotein isolation and enrichment
Keywords: Phosphoprotein; Chromatography; IMAC; Chromatofocusing; Immunoprecipitation
Copper-binding activity of Trefoil factor 1 (TFF1): A new perspective in the study of the multifunctional roles of TFFs
Keywords: ESI-MS; electrospray ionization-mass spectrometry; SELDI-TOF; surface enhanced laser desorption/ionization time-of-flight; LC-MS; liquid chromatography-mass spectrometry; IMAC; immobilized metal affinity chromatography; Trefoil factor; TFF1; Copper-bi
A novel method to quantify sphingosine 1-phosphate by immobilized metal affinity chromatography (IMAC)
Keywords: HPLC; high-performance liquid chromatography; HUVEC; human umbilical vein endothelial cells; IMAC; immobilized metal affinity chromatography; MEEC; mouse embryonic endothelial cells; MEF; mouse embryonic fibroblast; S1P; sphingosine 1-phosphate; S1Px; sph
Differential expression of the Ebola virus GP1, 2 protein and its fragments in E. coli
Keywords: Ebola virus; Glycoprotein; E. coli expression; IMAC; Refolding
Differential interactions of plasmid DNA, RNA and endotoxin with immobilised and free metal ions
Keywords: Plasmid DNA vaccine; RNA; Endotoxin; IMAC; Metal ion precipitation
Expression of functional human glutaminase in baculovirus system: Affinity purification, kinetic and molecular characterization
Keywords: GA; phosphate-activated glutaminase; KGA; kidney-type glutaminase isoform; LGA; liver-type glutaminase isoform; GIP; glutaminase-interacting protein; IMAC; immobilized metal affinity chromatography; MPP; mitochondrial processing peptidase; Human isozymes;
Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene
Keywords: SARS-CoV; Nucleocapsid; E. coli expression; IMAC; Refolding
Neutral additives enhance the metal-chelate affinity adsorption of nucleic acids: Role of water activity
Keywords: Neutral solutes; RNA; DNA; Metal-chelate affinity; Ethanol; Adsorption; Isotherm; IMAC;
Characterisation of metal–chelate methacrylate monoliths
Keywords: Monoliths; CIM; Metal–chelate; IMAC; Chromatographic purification
High-throughput protein purification using an automated set-up for high-yield affinity chromatography
Keywords: High-throughput; Protein purification; IMAC; Recombinant protein; Proteomics;
Presence of imidazole in loading buffer prevents formation of free radical in immobilized metal affinity chromatography and dramatically improves the recovery of herpes simplex virus type 1 gene therapy vectors
Keywords: HSV-1 vector; IMAC; Purification; Inactivation; Cobalt; Tag; Free radical; Electron spin resonance; Imidazole
Protein digestion and phosphopeptide enrichment on a glass microchip
Keywords: Microchip; Phosphopeptide; Mass spectrometry; Protein digestion; IMAC
Phosphoproteins analysis in plants: A proteomic approach
Keywords: Medicago truncatula; Arabidopsis thaliana; Phosphoproteins; IMAC; Mass spectrometry;
Chemically modified carbon nanotubes as material enhanced laser desorption ionisation (MELDI) material in protein profiling
Keywords: Carbon nanotubes; MALDI; Protein profiling; Elution; IMAC; Biomarker
Reversible resolution of flavin and pterin cofactors of His-tagged Escherichia coli DNA photolyase
Keywords: FAD, flavin adenine dinucleotide; FADox, oxidized FAD; FADH, neutral FAD radical; FADH−, reduced anionic FAD; MTHF, methenyltetrahydrofolate; CPD, cyclobutane pyrimidine dimer; DTT, dithiothreitolApoflavoprotein; Cofactor binding; Flavin; IMAC; Photolyase
Evaluation of IDA-PEVA hollow fiber membrane metal ion affinity chromatography for purification of a histidine-tagged human proinsulin
Keywords: Proinsulin-(His)6; Purification; Affinity membrane; IMAC
Effect of number of poly(His) tags on the adsorption of engineered proteins on immobilized metal affinity chromatography adsorbents
Keywords: IMAC; Adsorption isotherm; Protein purification; Langmuir–Freundlich
Deglycosylation to obtain stable and homogeneous Pichia pastoris-expressed N–A1 domains of carcinoembryonic antigen
Keywords: Carcinoembryonic antigen; CEA; Pichia pastoris; IMAC; Concanavalin A; Endoglycosydase H; scFv
Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
Keywords: Affinity purification; Affinity tag; His-tag; IMAC; Recombinant protein; Protein purification; Tag removal; Endoproteases; Exopeptidases; Aminopeptidases; Therapeutic protein purification
Influence of the protein oligomericity on final yield after affinity tag removal in purification of recombinant proteins
Keywords: Immobilized metal affinity chromatography; IMAC; Tumour necrosis factor alpha; Histidine tags; Affinity tags; Proteases; Tag removal; Oligomeric proteins
Effect of the matrix structure and concentration of polymer-immobilized Ni2+–iminodiacetic acid complexes on retention of IgG1
Keywords: Matrix; Structure; IMAC; Sorption; Imidazole; IgG1; Model protein; Frontal chromatography
Effect of chemical modification of histidines on the copper-induced oligomerization of jack bean urease (EC 3.5.1.5)
Keywords: Urease; Canavalia ensiformis; Oligomerization; Diethylpyrocarbonate; Copper; Insecticide; Histidine; Dysdercus peruvianus; Inactivation; IMAC;
High-level expression and purification of recombinant horseradish peroxidase isozyme C in SF-9 insect cell culture
Keywords: Peroxidase isozyme C; Insect cell; Spodoptera frugiperda Sf-9; Expression; IMAC; Purification;
Expression, purification and characterization of recombinant (E)-β-farnesene synthase from Artemisia annua
Keywords: β-FS; β-farnesene synthase; FPP; farnesyl diphosphate; GPP; geranyl diphosphate; IMAC; immobilized metal affinity chromatography; IPTG; isopropyl thio-β-d-thiogalactoside; Artemisia annua; β-Farnesene synthase; cDNA cloning; GC-MS; Recombinant expre
Expression of recombinant MDA-BF-1 with a kinase recognition site and a 7-histidine tag for receptor binding and purification
Keywords: Bone metastasis-related factor-1; Glycoprotein; Insect cell expression system; Receptor; PKA; Affinity chromatography; IMAC;
Purification and characterization of a periplasmic laccase produced by Sinorhizobium meliloti
Keywords: Laccase; Sinorhizobium meliloti; IMAC; Characterization;
Evaluation of immobilized metal-ion affinity chromatography for the fractionation of natural Cu complexing ligands
Keywords: IMAC; Copper; Ligands; Soil solution;