کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1215493 | 966975 | 2008 | 10 صفحه PDF | دانلود رایگان |
The purification of IgG from human plasma was studied by comparing two affinity membranes complexed with Ni(II), prepared by coupling iminodiacetic acid (IDA) and Tris(2-aminoethyl)amine (TREN) to poly(ethylenevinyl alcohol), PEVA, hollow fiber membranes. The Ni(II)-TREN-PEVA hollow fiber membrane had lower capacity for human IgG than the complex Ni(II)-IDA-PEVA, but with similar selectivity. The IgG in peak fractions eluted from the Ni(II)-IDA-PEVA with a stepwise concentration gradient of Tris–HCl pH 7.0 (100–700 mM) reached a purity of 98% (based on IgG, IgM, IgA, albumin, and transferrin nephelometric analysis). Adsorption IgG data at different temperatures (4–37 °C) were analyzed using Langmuir model resulting in a calculated maximum capacity at 25 °C of 204.6 mg of IgG/g of dry membrane. Decrease in Kd with increasing temperature (1.7 × 10−5 to 5.3 × 10−6 M) indicated an increase in affinity with increased temperature. The positive value of enthalpy change (26.2 kJ/mol) indicated that the adsorption of IgG in affinity membrane is endothermic. Therefore, lower temperature induces adsorption as verified experimentally.
Journal: Journal of Chromatography B - Volume 861, Issue 1, 1 January 2008, Pages 64–73