کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1205987 | 965215 | 2009 | 12 صفحه PDF | دانلود رایگان |
To improve our knowledge on protein targets of uranyl ion (UO22+), we set up a proteomic strategy based on immobilized metal-affinity chromatography (IMAC). The successful enrichment of UO22+-interacting proteins from human kidney-2 (HK-2) soluble cell extracts was obtained using an ion-exchange chromatography followed by a dedicated IMAC process previously described and designed for the uranyl ion. By mass spectrometry analysis we identified 64 proteins displaying varied functions. The use of a computational screening algorithm along with the particular ligand-based properties of the UO22+ ion allowed the analysis and categorization of the protein collection. This profitable approach demonstrated that most of these proteins fulfill criteria which could rationalize their binding to the UO22+-loaded phase. The obtained results enable us to focus on some targets for more in-depth studies and open new insights on its toxicity mechanisms at molecular level.
Journal: Journal of Chromatography A - Volume 1216, Issue 28, 10 July 2009, Pages 5365–5376