Production and purification of recombinant human hepcidin-25 with authentic N and C-termini
Keywords: Hepcidin; P. pastoris; Recombinant expression; Expanded bed adsorption; IMAC
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Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)
Keywords: MIH; molt-inhibiting hormone; VTG; vitellogenin; CHH; crustacean hyperglycemic hormone; VIH; vitellogenesis-inhibiting hormone; MOIH; mandibular organ inhibiting hormone; ITP; ion transport peptide; IMAC; immobilized metal ion affinity chromatography; ANO
Molecular cloning, gene expression analysis, and recombinant protein expression of novel silk proteins from larvae of a retreat-maker caddisfly, Stenopsyche marmorata
Keywords: Caddisfly; Trichoptera; Stenopsyche marmorata; Silk protein; Aquatic silk; Smsp; DDBJ; DNA data bank of Japan; H-fibroin; fibroin heavy chain; HRP; horseradish peroxidase; IMAC; immobilized metal ion affinity chromatography; IPTG; isopropyl β-d-1-thiogal
A fast and simple method to eliminate Cpn60 from functional recombinant proteins produced by E. coli Arctic Express
Keywords: Recombinant protein; Arctic Express; Cpn60; IMAC
Immobilization of Bacillus subtilis oxalate decarboxylase on a Zn-IMAC resin
Keywords: Oxalate decarboxylase; OxDC; IMAC; Electron paramagnetic resonance; EPR;
Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions
Keywords: Immunoglobulin Y; Adsorption isotherms; Thermodynamic parameters; Cryogel; IMAC;
Dissection and engineering of the Escherichia coli formate hydrogenlyase complex
Keywords: BN-PAGE; blue native polyacrylamide gel electrophoresis; IMAC; immobilized metal affinity chromatography; Fermentation; Formate hydrogenlyase; [NiFe]-hydrogenase; Mutagenesis; Genetic engineering; Escherichia coli;
Reversible adsorption of catalase onto Fe3Â + chelated poly(AAm-GMA)-IDA cryogels
Keywords: Catalase; Cryogel; IMAC; IDA;
Functional roles in S-adenosyl-l-methionine binding and catalysis for active site residues of the thiostrepton resistance methyltransferase
Keywords: Ala; alanine; Arg; arginine; Asn; asparagine; Asp; aspartic acid; ESI-MS; electrospray ionization mass spectrometry; IMAC; immobilized metal ion affinity chromatography; IPTG; isopropyl β-D-1-thiogalactopyranoside; ITC; isothermal titration calorimetry;
An optimized coupled assay for quantifying diaminopimelate decarboxylase activity
Keywords: Assay development; Diaminopimelate decarboxylase; Lysine biosynthesis; Enzyme kinetics; Saccharopine dehydrogenase; CV; column volume; DAP; diaminopimelate; DAPDC; diaminopimelate decarboxylase; DTT; dithiothreitol; FDA; Federal Drug Administration; His6;
Iron-binding peptides from whey protein hydrolysates: Evaluation, isolation and sequencing by LC–MS/MS
Keywords: Iron complexes; Enzymatic hydrolysis; IMAC; Mass spectrometry; In vitro gastric digestion; Food supplement
Production and characterization of monospecific and bispecific antibodies against dengue virus NS1 protein
Keywords: Dengue; NS1; Monoclonal antibody; Quadroma; BSA; bovine serum albumin; bsMab; bispecific monoclonal antibodies; DHF; dengue hemorrhagic fever; ECL; enhanced chemiluminescence; ELISA; enzyme linked immunosorbent assay; FBS; fetal bovine serum; FITC; fluore
Characterization of acetohydroxyacid synthase from the hyperthermophilic bacterium Thermotoga maritima
Keywords: AHAS; acetohydroxyacid synthase; BCAA; branched chain amino acid; CCE; crude cell extract; CFE; cell-free extract; HTCCE; heat-treated crude cell extract; IB; inclusion body; ilv; isoleucine, leucine, valine; IMAC; immobilized metal affinity chromatograph
Free-radical first responders: The characterization of CuZnSOD and MnSOD regulation during freezing of the freeze-tolerant North American wood frog, Rana sylvatica
Keywords: ALS; amyotrophic lateral sclerosis; AMPK; 5â² adenosine monophosphate-activated protein kinase; Cm; the denaturant concentration at which half of the protein is unfolded; CuZnSOD; copper and zinc containing SOD; DEAE+; diethylaminoethyl cellulose; DEPC;
Imidazole-free purification of His3-tagged recombinant proteins using ssDNA aptamer-based affinity chromatography
Keywords: DNA aptamers; SELEX; Histidine tag; IMAC; Imidazole;
High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires
Keywords: ELISA; enzyme-linked immunosorbent assay; IMAC; immobilized metal ion affinity chromatography; InlA; Internalin A; InlB; Internalin B; IPTG; isopropyl β-D-1-thiogalactopyranoside; LAC; llama-alpaca-camel; LRR; leucine-rich repeat; MWCO; molecular weight
Optimising methods for the preservation, capture and identification of ubiquitin chains and ubiquitylated proteins by immunoblotting
Keywords: Ubiquitin; Ubiquitin-binding-domain; Immunoblotting; Deubiquitylase; Ubiquitin chain topology; DMP; dimethyl pimelidinate; DUB; deubiquitylase; GST; glutathione S-transferase; HEK; human embryonic kidney; IAA; iodoacetamide; IL-1R; IL-1 receptor; IMAC; im
Insights into the coiled-coil organization of the Hendra virus phosphoprotein from combined biochemical and SAXS studies
Keywords: HeV; Hendra virus; NiV; Nipah virus; N; nucleoprotein; L; large protein; P; phosphoprotein; IDP; intrinsically disordered protein; IDR; intrinsically disordered region; PNT; P N-terminal domain; PCT; P C-terminal domain; PMD; P multimerization domain; XD;
The human rhabdomyosarcoma cell line TE671 - Towards an innovative production platform for glycosylated biopharmaceuticals
Keywords: A1AT; alpha-1-antitrypsin; FCS; fetal calf serum; GlcNAc; N-acetylglucosamine; GM-CSF; granulocyte-macrophage colony-stimulating factor; IMAC; immobilized metal ion affinity chromatography; MALDI-TOF; matrix-assisted laser desorption/ionization time-of-
Recombinant production of Epstein-Barr virus BZLF1 trans-activator and characterization of its DNA-binding specificity
Keywords: EBV; ZEBRA; ZREs; Auto-induction; IMAC; Membrane adsorber;
Elevated antibody reactivity to measles virus NCORE protein among patients with multiple sclerosis and their healthy siblings with intrathecal oligoclonal immunoglobulin G production
Keywords: MS; multiple sclerosis; OCB; oligoclonal bands; MS OCB trait; multiple sclerosis oligoclonal band trait; IgG; immunoglobulin G; CSF; cerebrospinal fluid; NCORE; N-terminal portion of the measles nucleocapsid protein; CNS; central nervous system; MRZ; meas
Enhanced detection and desalting free protocol for phosphopeptides eluted from immobilized Fe (III) affinity chromatography in direct MALDI TOF analysis
Keywords: IMAC; Phosphopeptide sequential elution; Chelator competition; Signal enhancement; Desalting free;
Metal complexes of new bis(tacn) ligands: Syntheses and structures of copper(II) complexes
Keywords: Copper(II) complex; Aza macrocycle; Crystal structure; m-Xylene linker; IMAC; Bis(tacn) ligand
Production of fully assembled and active Aquifex aeolicus F1FO ATP synthase in Escherichia coli
Keywords: IPTG; isopropyl-β-thiogalactopyranoside; DM; n-decyl-β-d-maltoside; DDM; n-dodecyl-β-d-maltoside; α-PCC; trans-4-(trans-4â²-propylcyclohexyl)cyclohexyl-α-d-maltoside; BN-PAGE; blue native polyacrylamide gel electrophoresis; IMAC; immobilized-metal a
The beta-isoform of the BRCA2 and CDKN1A(p21)-interacting protein (BCCIP) stabilizes nuclear RPL23/uL14
Keywords: BCCIP; BRCA2 and CDKN1A(p21,CIP1)-interacting protein; TAP; tandem affinity purification; IMAC; immobilized metal affinity chromatography; BCCIP; Ribosome biogenesis; Eukaryotic initiation factor 6 (eIF6); Ribosomal protein; RPL23/uL14;
Detection and molecular cloning of CYP74Q1 gene: Identification of Ranunculus acris leaf divinyl ether synthase
Keywords: DES; divinyl ether synthase; RaDES; Ranunculus acris divinyl ether synthase; HPL; hydroperoxide lyase; AOS; allene oxide synthase; 13(S)-HPOT; (9Z,11E,13S,15Z)-13-hydroperoxyoctadecatrienoic acid; 13(S)-HPOD; (9Z,11E,13S)-13-hydroperoxyoctadecadienoic aci
Protein complex purification from Thermoplasma acidophilum using a phage display library
Keywords: EM; electron microscopy; IMAC; immobilized metal affinity chromatography; MS/MS; tandem mass spectrometry; Ni-NTA; nickel-nitriloacetic acid; OHA-300; 300Â mM K2HPO4-KH2PO4 elution fraction of Sup12-HMWF; OHA-500; 500Â mM K2HPO4-KH2PO4 elution fra
A CSPG4-specific immunotoxin kills rhabdomyosarcoma cells and binds to primary tumor tissues
Keywords: CSPG4; chondroitin sulfate proteoglycan 4; ETAâ²; truncated version of Pseudomonas exotoxin A; IMAC; ion metal affinity chromatography; IT; immunotoxin; MCSP; Melanoma-associated chondroitin sulfate proteoglycan; RMS; rhabdomyosarcoma; aRMS; alveolar RMS
Expression of the [FeFe] hydrogenase in the chloroplast of Chlamydomonas reinhardtii
Keywords: [FeFe] hydrogenase; Chlamydomonas; Chloroplast; Gene expression; Green algae; Chl; chlorophyll; EDTA; ethylenediamine tetraacetic acid; IMAC; immobilized metal affinity chromatography; PAR; photosynthetically active radiation; TPP; thiamine pyrophosphate;
Separation of human Fab fragments on negative mode Ni(II)-TREN-agarose chromatography
Keywords: Human Fab fragments; Soybean proteins; IMAC; Purification; Ni(II)-TREN
A novel bispecific antibody targeting tumor necrosis factor α and ED-B fibronectin effectively inhibits the progression of established collagen-induce arthritis
Keywords: RA; rheumatoid arthritis; CIA; collagen-induced arthritis; HSA; human serum albumin; HRP; horseradish peroxidase; TNF; tumor necrosis factor; TNF-scFv; anti-TNF-α scFv; CD3-scFv; anti-CD3 scFv; IMAC; immobilized-metal affinity chromatography; MTT; 3-(4,5
High-level expression in Escherichia coli, purification and kinetic characterization of Plasmodium falciparum M1-aminopeptidase
Keywords: Expression in Escherichia coli; IMAC; M1-family aminopeptidase; Malaria; PfA-M1; Plasmodium falciparum
Nuclear phosphoproteome of developing chickpea seedlings (Cicer arietinum L.) and protein-kinase interaction network
Keywords: Nuclear phosphopreoteome; IMAC; Pro-Q diamond; Mass spectrometry; Non-model plant; Protein-kinase interactome
Do-it-yourself histidine-tagged bovine enterokinase: A handy member of the protein engineer's toolbox
Keywords: BENAC; benzamidine affinity chromatography; EK; enterokinase; GST; glutathione-S-transferase; IB; inclusion body; IEC; ion exchange chromatography; IMAC; mmobilized metal ion affinity chromatography; KLK; kallikrein-related peptidase; SUMO; small ubiquiti
Purification and characterization of caclium-binding soybean protein hydrolysates by Ca2+/Fe3+ immobilized metal affinity chromatography (IMAC)
Keywords: Purification; Characterization; Soybean protein hydrolysates; Calcium binding; Amino acid composition; IMAC;
A high-throughput assay for the detection of Tyr-phosphorylated proteins in urine of bladder cancer patients
Keywords: TPPs; Tyr-phosphorylated proteins; IMAC; immobilized metal affinity chromatography; SCC; standard column chromatography; MIP; miniaturized IMAC procedure; AU; arbitrary units; SU; standard units; AUC; area under the ROC curve; Phosphotyrosine proteins; Ur
Structural and functional characterization of the single-chain Fv fragment from a unique HCV E1E2-specific monoclonal antibody
Keywords: CDR; complementarity-determining region; DAAs; direct-acting antivirals; ELISA; enzyme-linked immunoabsorbent assay; FR; framework region; GT; genotype; HCV; hepatitis C virus; HCVsp; serum-derived HCV particles; HRP; horseradish peroxidase; IgG; immunogl
Easy expression of the C-terminal heavy chain domain of botulinum neurotoxin serotype A as a vaccine candidate using a bi-cistronic baculovirus system
Keywords: ACh; acetylcholine; AcMNPV; Autographa californica multiple polyhedrosis virus; BEVS; baculovirus expression vector system; BoNT/A; botulinum toxin A; EGFP; enhanced green fluorescent protein; ELISA; enzyme-linked immunosorbent assay; HC; heavy chain; IMA
The novel purification and biochemical characterization of a reversible CYP24A1:adrenodoxin complex
Keywords: P450 or CYP; cytochrome P450; CYP24A1; cytochrome P450 24A1; 25(OH)D3; 25-hydroxyvitamin-D3; 1,25(OH)2D3; 1α,25-dihydroxyvitamin-D3; 1,25(OH)2D2; 1α,25-dihydroxyvitamin-D2; Adx; adrenodoxin; CHAPS; 3-[(3-cholamidopropyl)dimethylammonio]-2-hydroxy-1-prop
Indoleamine 2,3-dioxygenase inhibitory activity of derivatives of marine alkaloid tsitsikammamine A
Keywords: IDO1; indoleamine 2,3-dioxygenase; TDO; tryptophan 2,3-dioxygenase; NHDF; normal fibroblast cell lines; MTT; (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; EDTA; ethylenediaminetetraacetic acid; IMAC; immobilized metal ion affinity chromat
Phosphoproteomic analysis of the striatum from pleiotrophin knockout and midkine knockout mice treated with cocaine reveals regulation of oxidative stress-related proteins potentially underlying cocaine-induced neurotoxicity and neurodegeneration
Keywords: NT5C; 5â²(3â²)-deoxyribonucleotidase; ERP29; endoplasmic reticulum resident protein 29; ERP60; endoplasmic reticulum resident protein 60; ERK; extracellular signalregulated kinase; GLUD1; glutamate dehydrogenase 1; HBB1; hemoglobin subunit beta-1; HBB2;
Detection of soluble co-factor dependent protein expression in vivo: Application to the 4â²-phosphopantetheinyl transferase PptT from Mycobacterium tuberculosis
Keywords: ACP; acyl carrier protein; AcpS; acyl carrier protein synthase; AnTet; anhydrotetracycline; BSA; bovine serum albumin; CoA; Coenzyme A; DHFR; dihydrofolate reductase; DSF; differential scanning fluorimetry; DTT; dithiothreitol; GFP; green fluorescent prot
Correlation profiling for determining kinase-substrate relationships
Keywords: ACN; acetonitrile; AEX; anion exchange chromatography; AGC; automatic gain control; Ambic; ammonium bicarbonate; ATP; adenosine triphosphate; AS; asynchronous; CID; collision induced dissociation; DTT; dithiothreitol; EDTA; ethylene diamine tetra-acetic a
Differential phosphoproteome of the striatum from pleiotrophin knockout and midkine knockout mice treated with amphetamine: Correlations with amphetamine-induced neurotoxicity
Keywords: ALDH1A1; aldehyde dehydrogenase family 1 member A1; ANXA7; annexin A7; ATP5A1; ATP synthase subunit alpha; ATP5D; ATP synthase subunit d; COPS5; COP9 signalosome subunit 5; CKMT1; creatine kinase U-type; ALAD; delta-aminolevulinic acid dehydratase; ERP29;
High level prokaryotic expression of anti-Müllerian inhibiting substance type II receptor diabody, a new recombinant antibody for in vivo ovarian cancer imaging
Keywords: 12G4Db; dimeric diabody 12G4; 12G4Fm; monomeric format 12G4; Arg; arginine; BSA; bovine serum albumin; DTT; dithiothreitol; Gu-HCl; guanidine-HCl; IMAC; immobilized metal ion affinity chromatography; IPTG; isopropyl-β-d-thiogalactopyranoside; mAb; monocl
Functional properties of cell-free expressed human endothelin A and endothelin B receptors in artificial membrane environments
Keywords: Aso-PC; soybean l-α-phosphatidylcholine; BTE; brain total extract; CECF; continuous exchange cell-free; CF; cell-free; CHS; cholesteryl hemisuccinate; CVs; column volumes; DMPC; 1,2-dimyristoyl-sn-glycero-3-phosphocholine; DMPG; 1,2-dimyristoyl-sn-glycer
Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation
Keywords: ACN; acetonitrile; AGC; automatic gain control; CID; collision induced dissociation; CREB; 3â²-5â²-cyclic adenosine monophosphate response element-binding protein; CRTC2; CREB regulated transcription coactivator 2; DTT; dithiothreitol; FA; formic acid;
Synthesis and characterization of amino acid containing Cu(II) chelated nanoparticles for lysozyme adsorption
Keywords: Lysozyme; Nanoparticles; IMAC; Tryptophan
Purification of yeast alcohol dehydrogenase by using immobilized metal affinity cryogels
Keywords: Alcohol dehydrogenase; Yeast; Cryogel; IMAC; Iminodiacetic acid
Arrayed antibody library technology for therapeutic biologic discovery
Keywords: CDR; complementary determining region; mAb; monoclonal antibody; V; variable; D; diversity; J; joining; GPCR; G-protein coupled receptor; CVB; culture vessel block; LIMS; laboratory information management system; Fab; fragment antigen-binding; SAR; struct