کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8348015 | 1541711 | 2015 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential regulation of hepatopancreatic vitellogenin (VTG) gene expression by two putative molt-inhibiting hormones (MIH1/2) in Pacific white shrimp (Litopenaeus vannamei)
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کلمات کلیدی
VitellogeninORFsMIHDulbecco Modified Eagle Mediumisopropyl-β-d-1-thiogalactopyranosideTBSTVitellogenesisCHHVIHHBSSVitellogenesis-inhibiting hormoneBCAIMACIPTGPVDFDMEMGSIIon transport peptideTris-buffered saline with 0.05% Tween 20Hanks balanced salt solution - Hanks محلول نمک را تعویض می کندHepatopancreas - hepatopancreasEDTA - اتیلن دی آمین تترا استیک اسید ethylene diamine tetraacetic acid - اتیلن دیامین تتراستیک اسیدbicinchoninic acid - بیسینکنینیک اسیدanalysis of variance - تحلیل واریانسANOVA - تحلیل واریانس Analysis of varianceReproduction - تولید مثل یا زادآوریpolyvinylidene difluoride - دی فلوئورید پلی وینیلیدینgonadosomatic index - شاخص گنادوسوماتیکOpen reading frames - فریم های خواندن را باز کنیدLitopenaeus vannamei - میگوی سفید غربی، لیتوپنئوس وانامیMolt-inhibiting hormone - هورمون بسیار مهار کنندهCrustacean Hyperglycemic Hormone - هورمون هیپرگلیسمی CrustaceanITP - و غیره.vtg - ویتگimmobilized metal ion affinity chromatography - کروماتوگرافی جذب یون فلز بی حرکتی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Molt-inhibiting hormone (MIH), a peptide member of the crustacean hyperglycemic hormone (CHH) family, is commonly considered as a negative regulator during the molt cycle in crustaceans. Phylogenetic analysis of CHH family peptides in penaeidae shrimps suggested that there is no significant differentiation between MIH and vitellogenesis-inhibiting hormone (VIH, another peptide member of CHH family), by far the most potent negative regulator of crustacean vitellogenesis known. Thus, MIH may also play a role in regulating vitellogenesis. In this study, two previously reported putative MIHs (LivMIH1 and LivMIH2) in the Pacific white shrimp (Litopenaeus vannamei) were expressed in Escherichia coli, purified by immobilized metal ion affinity chromatography (IMAC) and further confirmed by western blot. Regulation of vitellogenin (VTG) mRNA expression by recombinant LivMIH1 and LivMIH2 challenge was performed by both in vitro hepatopancreatic primary cells culture and in vivo injection approaches. In in vitro primary culture of shrimp hepatopancreatic cells, only LivMIH2 but not LivMIH1 administration could improve the mRNA expression of VTG. In in vivo injection experiments, similarly, only LivMIH2 but not LivMIH1 could stimulate hepatopancreatic VTG gene expression and induce ovary maturation. Our study may provide evidence for one isoform of MIH (MIH2 in L. vannamei) may serve as one of the mediators of the physiological progress of molting and vitellogenesis. Our study may also give new insight in CHH family peptides regulating reproduction in crustaceans, in particular penaeidae shrimps.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 68, June 2015, Pages 58-63
Journal: Peptides - Volume 68, June 2015, Pages 58-63
نویسندگان
Xing Luo, Ting Chen, Ming Zhong, Xiao Jiang, Lvping Zhang, Chunhua Ren, Chaoqun Hu,