کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5859330 | 1132469 | 2013 | 8 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Phosphoproteomic analysis of the striatum from pleiotrophin knockout and midkine knockout mice treated with cocaine reveals regulation of oxidative stress-related proteins potentially underlying cocaine-induced neurotoxicity and neurodegeneration
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کلمات کلیدی
PTNglutamate dehydrogenase 1GLUD1RPTPPRDX6ERp292D-PAGEMidkineERKIMACMALDI-TOFtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیParkinson's disease - بیماری پارکینسونOxidative stress - تنش اکسیداتیوPhosphoproteomics - فسفوپروتومیکMatrix-Assisted Laser Desorption/Ionization Time-of-Flight - مدت زمان پرواز یونیزاسیون لیزر ماتریکس کمک می کندwild type - نوع وحشیReceptor protein tyrosine phosphatase - پروتئین گیرنده تیروزین فسفاتازPleiotrophin - پلئوتروفینimmobilized metal affinity chromatography - کروماتوگرافی وابسته به فلز متمرکز
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The neurotrophic factors pleiotrophin (PTN) and midkine (MK) are highly upregulated in different brain areas relevant to drug addiction after administrations of different drugs of abuse, including psychostimulants. We have previously demonstrated that PTN and MK modulate amphetamine-induced neurotoxicity and that PTN prevents cocaine-induced cytotoxicity in NG108-15 and PC12 cells. In an effort to dissect the different mechanisms of action triggered by PTN and MK to exert their protective roles against psychostimulant neurotoxicity, we have now used a proteomic approach to study protein phosphorylation, in which we combined phosphoprotein enrichment, by immobilized metal affinity chromatography (IMAC), with two-dimensional gel electrophoresis and mass spectrometry, in order to identify the phosphoproteins regulated in the striatum of PTN knockout, MK knockout and wild type mice treated with a single dose of cocaine (15Â mg/kg, i.p.). We identified 7 differentially expressed phosphoproteins: 5â²(3â²)-deoxyribonucleotidase, endoplasmic reticulum resident protein 60 (ERP60), peroxiredoxin-6 (PRDX6), glutamate dehydrogenase 1 (GLUD1), aconitase and two subunits of hemoglobin. Most of these proteins are related to neurodegeneration processes and oxidative stress and their variations specially affect the PTN knockout mice, suggesting a protective role of endogenous PTN against cocaine-induced neural alterations. Further studies are needed to validate these proteins as possible targets against neural alterations induced by cocaine.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 314, Issue 1, 6 December 2013, Pages 166-173
Journal: Toxicology - Volume 314, Issue 1, 6 December 2013, Pages 166-173
نویسندگان
Marta Vicente-RodrÃguez, Esther Gramage, Gonzalo Herradón, Carmen Pérez-GarcÃa,