کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
10825973 | 1064692 | 2013 | 28 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation
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کلمات کلیدی
CREBPMSFCRTC2CIDAGCAcOHTFAHEPESFDRIMACXICDTTACN(4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) - (4- (2-hydroxyethyl) -1-piperazineethanesulfonic acid)2-(N-morpholino) ethanesulfonic acid - 2- (N-مورفولینو) اتان سولفونیک اسیدLC–MS/MS - LC-MS / MSm/z - m / zS/N - S / NAcetonitrile - استونیتریلscx - اسکاTrifluoroacetic acid - اسید TrifluoroaceticAcetic acid - اسید استیکFormic acid - اسید فرمیکstrong cation exchange - تبادل کاتیون قویcollision induced dissociation - تقارن ناشی از برخوردdithiothreitol - دیتیوتریتولSILAC - سیلکMass spectrometry - طیف سنجی جرمیPhosphorylation - فسفریلاسیونPhenylmethanesulfonyl fluoride - فنیل متیل سولفونیل فلورایدMeS - مسfalse discovery rate - میزان کشف کاذبmass to charge ratio - نسبت جرم به شارژSignal-to-noise ratio - نسبت سیگنال به نویزstable isotope labeling by amino acids in cell culture - نشانه ایزوتوپ پایدار با اسیدهای آمینه در کشت سلولیQuantitative proteomics - پروتئومیک کمLiver - کبدliquid chromatography–tandem mass spectrometry - کروماتوگرافی مایع و اسپکترومتری توده دو طرفهimmobilized metal affinity chromatography - کروماتوگرافی وابسته به فلز متمرکزextracted ion chromatogram - کروماتوگرافی یون استخراج شدهautomatic gain control - کنترل اتوماتیک
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation Quantitative comparison of the fasted and re-fed mouse liver phosphoproteomes using lower pH reductive dimethylation](/preview/png/10825973.png)
چکیده انگلیسی
Phosphorylation is a common but crucial protein posttranslational modification occurring in virtually all known species. A successful technique for identifying phosphorylation sites is via liquid chromatography-tandem mass spectrometry (LC-MS/MS). In addition to identification, the introduction of stable isotopes allows for LC-MS based quantification of thousands of phosphorylation sites. Historically, stable isotope labeling by amino acids in cell culture (SILAC) has been the preferred method for introducing stable isotopes for quantification. SILAC is not well suited, however, for quantitative proteomics in larger animals. The introduction of stable isotope instead by reductive dimethylation is an alternative for performing quantitative proteomics in animal tissues. Here we present an improved reductive dimethylation protocol and demonstrate the application of this method in the analysis of the fasted vs. re-fed mouse liver phosphoproteome. In our analysis, greater than 8500 sites were identified from â¼2700 phosphoproteins. Nearly 7400 phosphorylation events from â¼2300 phosphoproteins were reliably quantified. Using a 2-fold change as a cutoff, 390 phosphorylation sites were found to change between fasted and re-fed mice, many of which may have interesting biological interpretations.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Methods - Volume 61, Issue 3, 15 June 2013, Pages 277-286
Journal: Methods - Volume 61, Issue 3, 15 June 2013, Pages 277-286
نویسندگان
Joshua T. Wilson-Grady, Wilhelm Haas, Steven P. Gygi,