کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
5859368 | 1562350 | 2013 | 10 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Differential phosphoproteome of the striatum from pleiotrophin knockout and midkine knockout mice treated with amphetamine: Correlations with amphetamine-induced neurotoxicity
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کلمات کلیدی
ENO2ALDH1A1ATP5A12D-PAGEATP synthase subunit alphaPGAM1PTNMidkineANXA7ERp29ERKAnnexin A7IMACRPTPMALDI-TOFAmphetamine - آمفتامین هاtwo-dimensional polyacrylamide gel electrophoresis - الکتروفورز ژل پلی آکریل آمید دو بعدیParkinson's disease - بیماری پارکینسونdelta-aminolevulinic acid dehydratase - دلتا آمینولولولین اسید دهیدراتازRån - شتمFah - فاphosphoproteins - فسفوپروتئین هاPhosphoglycerate mutase 1 - فسفوگلسرات ماتاز 1Matrix-Assisted Laser Desorption/Ionization Time-of-Flight - مدت زمان پرواز یونیزاسیون لیزر ماتریکس کمک می کندALAD - مناطقwild type - نوع وحشیProteomics - پروتئومیکسReceptor protein tyrosine phosphatase - پروتئین گیرنده تیروزین فسفاتازPleiotrophin - پلئوتروفینimmobilized metal affinity chromatography - کروماتوگرافی وابسته به فلز متمرکزextracellular signal-regulated kinase - کیناز تنظیم شده سیگنال خارج سلولی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم محیط زیست
بهداشت، سم شناسی و جهش زایی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The neurotrophic factors pleiotrophin (PTN) and midkine (MK) have been shown to modulate amphetamine-induced neurotoxicity. Accordingly, PTNâ/â and MKâ/â mice show an increased vulnerability to amphetamine-induced neurotoxic effects. In an effort to uncover new pharmacological targets to prevent amphetamine neurotoxic effects, we have now used a proteomic approach to study protein phosphorylation, in which we combined phosphoprotein enrichment, by immobilized metal affinity chromatography (IMAC), with two-dimensional gel electrophoresis and mass spectrometry, in order to identify the phosphoproteins regulated in the striatum of PTNâ/â, MKâ/â and wild type (WT) mice treated with amphetamine. We identified 13 differentially expressed phosphoproteins that are judged to be relevant in the neuroprotective roles of PTN and MK against amphetamine-induced neurotoxicity. It is very interesting to note that 4 of these phosphoproteins, annexin A7 (ANXA7), COP9 signalosome subunit 5 (COPS5), aldehyde dehydrogenase family 1 member A1 (ALDH1A1) and creatine kinase U-type (CKMT1), are known to be involved in Parkinson's disease, a result of significant importance since PTN and MK have been also demonstrated to limit Parkinson's disease (PD) progress and have been suggested to be among the important genetic factors possibly preventing the development of PD in methamphetamine abusers. The data identify phosphoproteins differentially regulated by amphetamine treatment and/or the presence of endogenous PTN/MK which may be relevant mediators of PTN/MK neuroprotective effects against amphetamine-induced neurotoxicity. The data support further studies to validate the phosphoproteins here identified as possible new pharmacological targets to prevent amphetamine neurotoxic effects.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Toxicology - Volume 306, 5 April 2013, Pages 147-156
Journal: Toxicology - Volume 306, 5 April 2013, Pages 147-156
نویسندگان
Esther Gramage, Gonzalo Herradón, Yasmina B. MartÃn, Marta Vicente-RodrÃguez, Loreto Rojo, Heike Gnekow, Aurora Barbero, Carmen Pérez-GarcÃa,