کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
8360610 | 1542346 | 2014 | 5 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Recombinant production of Epstein-Barr virus BZLF1 trans-activator and characterization of its DNA-binding specificity
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
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![عکس صفحه اول مقاله: Recombinant production of Epstein-Barr virus BZLF1 trans-activator and characterization of its DNA-binding specificity Recombinant production of Epstein-Barr virus BZLF1 trans-activator and characterization of its DNA-binding specificity](/preview/png/8360610.png)
چکیده انگلیسی
This paper describes the recombinant production of a biologically active Epstein-Barr virus BZLF1 trans-activator, i.e., Z-encoded broadly reactive activator (ZEBRA), that recognized specific DNA motifs. We used auto-induction for histidine-tagged BZLF1 expression in Escherichia coli and immobilized cobalt affinity membrane chromatography for protein purification under native conditions. We obtained the purified BZLF1 at a yield of 5.4Â mg per gram of wet weight cells at 75% purity, in which 27% of the recombinant BZLF1 remained biologically active. The recombinant BZLF1 bound to oligonucleotides containing ZEBRA response elements, either AP-1 or ZIIIB, but not a ZIIIB mutant. The recombinant BZLF1 showed a specific DNA-binding activity which could be useful for functional studies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Protein Expression and Purification - Volume 95, March 2014, Pages 8-12
Journal: Protein Expression and Purification - Volume 95, March 2014, Pages 8-12
نویسندگان
Chun Shen Lim, Siang Ling Goh, Gopala Krishnan, Ching Ching Ng,