Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions

The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu2+ cryogel system was studied by batch equilibrium measurements. The adsorptive equilibrium data were obtained at 17 and 27 °C, pH 5.0 and 6.5, and at ionic strength of 50 and 200 mmol L−1 NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu2+ cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27 °C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27 mg g−1. The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.