کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
7611815 | 1493531 | 2015 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Adsorption of immunoglobulin Y in supermacroporous continuous cryogel with immobilized Cu2+ ions
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
The influence of temperature, pH and ionic strength on the adsorption of Immunoglobulin Y (IgY) in IDA-Cu2+ cryogel system was studied by batch equilibrium measurements. The adsorptive equilibrium data were obtained at 17 and 27 °C, pH 5.0 and 6.5, and at ionic strength of 50 and 200 mmol Lâ1 NaCl. Langmuir, Freundlich and Langmuir-Freundlich models were fitted to equilibrium data, while the enthalpy of adsorption of IgY in IDA-Cu2+ cryogel system was calculated through Van't Hoff analysis. The binding of IgY on cryogel was stronger at 27 °C and lowest pH and ionic strength values, with apparent maximum adsorption capacity of 27 mg gâ1. The adsorption of protein in the resin was spontaneous in all analyzed cases. The results provide valuable information to enable the improvement of IgY purification processes.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Chromatography A - Volume 1395, 22 May 2015, Pages 16-22
Journal: Journal of Chromatography A - Volume 1395, 22 May 2015, Pages 16-22
نویسندگان
Willer Ferreira da Silva Júnior, Rodrigo Cano, Antonio Helvécio Totola, Lorendane Milena de Carvalho, Marcel Otávio Cerri, Jane Sélia dos Reis Coimbra, Gleidson Giordano Pinto de Carvalho, Bruna Mara Aparecida de Carvalho,