کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
8417558 1545693 2015 10 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی بیوتکنولوژی یا زیست‌فناوری
پیش نمایش صفحه اول مقاله
High affinity anti-Internalin B VHH antibody fragments isolated from naturally and artificially immunized repertoires
چکیده انگلیسی
The need for rapid and easy technologies for the detection of food-borne and environmental pathogens is essential for safeguarding the health of populations. Furthermore, distribution of tainted food and water can have consequences which can affect whole economies. Antibodies and antibody fragments have been historically used in detection platforms due to their antigen specificity and robust physicochemical properties. In this study, we report the isolation and characterization of antibody fragments from the heavy chain antibody repertoire (VHH) of Camelidae which bind with specificity and high affinity to the Listeria monocytogenes invasin, Internalin B (InlB). To the best of our knowledge, this is the first report of anti-InlB VHHs from camelids. These anti-InlB VHHs were not cross-reactive to the structurally related Listeria invasin Internalin A (InlA) and are potential reagents to be used in the development of detection and medical technologies.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Immunological Methods - Volume 416, January 2015, Pages 29-39
نویسندگان
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