کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2008149 1066398 2007 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Gastrin 1–6 promotes growth of colon cancer cells through non-CCK receptors
موضوعات مرتبط
علوم زیستی و بیوفناوری بیوشیمی، ژنتیک و زیست شناسی مولکولی زیست شیمی
پیش نمایش صفحه اول مقاله
Gastrin 1–6 promotes growth of colon cancer cells through non-CCK receptors
چکیده انگلیسی

Our previous studies have shown that stimulation of proliferation of DLD-1 and HT29 human colonic cancer cells by nanomolar gastrin (G17) and carboxymethyl gastrin (G17Gly) and reversal of growth by micromolar G17 and G17Gly involves binding sites which can neither be CCK1 nor CCK2 receptors; the N terminal fragment, G17(1–12), is sufficient to increase the number of HT-29 cells by binding the higher affinity binding site but is without a suppressing effect through the lower affinity site. In this study with DLD-1 cells, competitive binding using 125I-G17(1–12) showed that G17(1–12) binds both high and low affinity sites, as do G17 and G17Gly. G17(1–6)-NH2, even without the central-to-C-terminal portion of G17, was still able to bind a single site and to promote a dose-dependent increase in cell number at nanomolar concentrations. The results indicate the presence of a non-CCK receptor on human colonic cancer cells which could mediate the tumor-promoting activity of the N-terminal-to-central portion of G17Gly which, unlike G17, is produced by such cells.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Peptides - Volume 28, Issue 3, March 2007, Pages 632–635
نویسندگان
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