کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2008547 | 1066422 | 2007 | 7 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis Sequence characterization and expression patterns of two defensin-like antimicrobial peptides from the tick Haemaphysalis longicornis](/preview/png/2008547.png)
Two cDNAs encoding defensin-like antimicrobial peptides were cloned and sequenced from the tick Haemaphysalis longicornis. The full-length cDNA of Hlgut-defensin (H. longicornis midgut defensin) is 333 bp, encoding an expected protein with 73 amino acids. The full-length cDNA of Hlsal-defensin (H. longicornis salivary gland defensin) is 382 bp, encoding an expected protein with 81 amino acids. The antibacterial activities of the synthetic peptides based on the Hlgut-defensin and Hlsal-defensin sequences were tested against a variety of Gram-positive and Gram-negative bacteria. Using real-time PCR, the tissue-specific expression of two defensin-like peptides were determined and it was also found that the gene transcripts of Hlgut-defensin and Hlsal-defensin were significantly induced by a lipopolysaccharide (LPS) injection.
Journal: Peptides - Volume 28, Issue 6, June 2007, Pages 1304–1310