کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2008713 | 1066439 | 2005 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Isolation and characterization of a glycine-extended form of calcitonin receptor-stimulating peptide-1: Another biologically active form of calcitonin receptor-stimulating peptide-1 Isolation and characterization of a glycine-extended form of calcitonin receptor-stimulating peptide-1: Another biologically active form of calcitonin receptor-stimulating peptide-1](/preview/png/2008713.png)
In this study, we isolated a peptide eliciting a potent stimulatory effect on cAMP production in LLC-PK1 cells from acid extracts of porcine brain. By structural analysis, this peptide was determined to be a C-terminal glycine-extended form of calcitonin receptor-stimulating peptide-1 (CRSP-1-Gly). Synthetic CRSP-1-Gly enhanced the cAMP production in COS-7 cells expressing calcitonin (CT) receptor as strongly as CRSP-1. Measurement of immunoreactive (IR) CRSP-1-Gly by radioimmunoassay using the specific antisera against CRSP-1-Gly showed that a relatively high level (>1 pmol/g wet weight) of IR-CRSP-1-Gly was detected in the midbrain, hypothalamus, anterior and posterior lobes of pituitary, and thyroid gland, and the ratio of IR-CRSP-1-Gly to total IR-CRSP-1 varies from 0.02 to 0.35 in each tissue. These results suggest that CRSP-1-Gly is actually present in the tissues as one of major endogenous molecular forms of CRSP-1, and can regulate the cells expressing the CT receptor both in the central nervous system and peripheral tissues in a manner similar to that of CRSP-1. IR-CRSP-2 and IR-CRSP-3 are also present in the brain and other tissues, but their tissue concentrations are 33% on average and less than 3% that of total IR-CRSP-1, respectively.
Journal: Peptides - Volume 26, Issue 12, December 2005, Pages 2616–2623