کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
2009090 1541779 2015 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Luteolin suppresses TCDD-induced wasting syndrome in a cultured adipocyte model
موضوعات مرتبط
علوم زیستی و بیوفناوری علوم کشاورزی و بیولوژیک علوم زراعت و اصلاح نباتات
پیش نمایش صفحه اول مقاله
Luteolin suppresses TCDD-induced wasting syndrome in a cultured adipocyte model
چکیده انگلیسی


• Luteolin suppressed TCDD-induced loss of lipid accumulation in 3T3-L1 adipocytes.
• Luteolin inhibited the TCDD-caused decrease in expression of PPARγ and C/EBPα.
• Luteolin canceled TCDD-caused decrease in the DNA binding of C/EBPβ and C/EBPδ.
• Luteolin was rapidly incorporated and accumulated in 3T3-L1 adipocytes.
• Thus, luteolin is a compound for the prevention of TCDD-induced wasting syndrome.

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) causes various toxic effects, including wasting syndrome, through activation of an aryl hydrocarbon receptor (AhR). Our previous report demonstrated that certain flavonoids inhibit the activation of AhR and suppress its DNA binding activity. In this study, we searched for an active compound among 13 flavonoids that suppressed TCDD-induced loss of lipid accumulation using 3T3-L1 adipocytes as a cell culture model for wasting syndrome. Two flavonoids, luteolin and epigallocatechin gallate, suppressed TCDD-induced loss of lipid accumulation in this model. We further investigated luteolin to clarify the underlying molecular mechanism and confirmed that luteolin inhibited nuclear translocation of AhR caused by TCDD. Luteolin also inhibited the TCDD-driven decrease in protein expression of peroxisome proliferator-activated receptor γ (PPARγ) and CCAAT/enhancer-binding protein α (C/EBPα). Although TCDD alone did not change protein expression of C/EBPβ and C/EBPδ, luteolin and TCDD up-regulated C/EBPδ expression in a dose-dependent manner. On the other hand, TCDD significantly decreased DNA binding of C/EBPβ and C/EBPδ, and luteolin completely canceled TCDD-decreased DNA binding of them. We conclude that luteolin suppresses the TCDD-induced loss of lipid accumulation in 3T3-L1 adipocytes by preventing a decrease in protein expression of PPARγ and C/EBPα, the master regulators of adipocyte differentiation and in DNA binding of C/EBPβ and C/EBPδ. Moreover, luteolin was rapidly incorporated and accumulated in 3T3-L1 adipocytes. Thus, luteolin is an attractive compound for the prevention of TCDD-induced wasting syndrome.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 120, May 2015, Pages 14–20
نویسندگان
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