PCR-RFLP methods for detection of insecticide resistance-associated mutations in the house fly (Musca domestica)

► A simple PCR-RFLP assay for reliably detecting G342A/V mutations in Ace was developed. ► A two-PCR-RFLP-assay protocol was described for genotyping SNPs (1014L/F/H) in Vssc. ► A primer-introduced restriction strategy was used in the detection of SNPs (251 W/L/S) in mdaE7.