PCR-based suppression subtractive hybridization analyses of enantioselective gene expression in root tips of wheat treated with optically active urea compounds

► R-/S-MBTU show dichotomic and enantioselective effects on Gramineae plants. ► S-MBTUs mode of action was explored using SSH and qRT-PCR technique. ► S-MBTU down-regulated gene expression of GS, AHAS, a-TUB2, and ACT, enantioselectively.