کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2010120 | 1066713 | 2010 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Enzymatic dynamics of catechol oxidase from Gastrolina depressa
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کلمات کلیدی
موضوعات مرتبط
علوم زیستی و بیوفناوری
علوم کشاورزی و بیولوژیک
علوم زراعت و اصلاح نباتات
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چکیده انگلیسی
Properties of the phenoloxidase (PO) from adult of Gastrolina depressa Baly (Coleoptera: Chrysomelidae) as well as effects of some metal ions and inhibitors on the activity of PO purified by (NH4)2SO4 were determined. The optimal pH and temperature of the enzyme for the oxidation of catechol were determined to be at pH 7.5 and at 40 °C, respectively. The kinetic parameters for the oxidation of L-DOPA and catechol by the PO were 15.01 and 9.17 mM, respectively. The PO activity was strongly inhibited by Zn2+ and Cu2+, different to Mg2+ slightly. Both ascorbic acid and cysteine exhibited competitive inhibition and the inhibitory constants (Ki) were determined to be 2.22 mM and 0.40 mM, respectively.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pesticide Biochemistry and Physiology - Volume 96, Issue 2, February 2010, Pages 57–62
Journal: Pesticide Biochemistry and Physiology - Volume 96, Issue 2, February 2010, Pages 57–62
نویسندگان
Yan Zhao, Chao-Bin Xue, Long Yang, Cheng-Gang Zhou, Wan-Chun Luo,