کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2010663 | 1066984 | 2016 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Embelin inhibits proliferation, induces apoptosis and alters gene expression profiles in breast cancer cells
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کلمات کلیدی
Embelin - امبلنEMT - تکنسین فوریتهای پزشکیApoptosis - خزان یاختهایKEGG یا Kyoto Encyclopedia of Genes and Genomes - دایرة المعارف ژن ها و ژنوم کیوتو Kyoto Encyclopedia of Genes and Genomes - دایره المعارف ژنتیک ژن ها و ژنوم کیوتوDAVID - دیویدBreast cancer - سرطان پستانgene expression profiling - مشخصات ژن بیانdatabase for annotation, visualization and integrated discovery - پایگاه داده برای حاشیه نویسی، تجسم و کشف یکپارچهEpithelial-mesenchymal transition - گذار اپیتلیال-مزانشیمی
موضوعات مرتبط
علوم زیستی و بیوفناوری
بیوشیمی، ژنتیک و زیست شناسی مولکولی
زیست شیمی
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
PurposeTo investigate effect of embelin on proliferation, apoptosis and gene expression profile changes in breast cancer cells.MethodsCell viability was determined by MTT assay and apoptosis assayed using flow cytometry. Differential expression of 84 genes commonly involved in breast cancer carcinogenesis was assessed by real-time PCR using the Human Breast Cancer RT2 Profiler PCR Array.ResultsMCF-7 and MDA-MB-231 cells were treated with embelin (0-25 μM) for 24 and 96 h. Embelin exhibited time and dose dependence in both cell lines and was more potent in inhibiting MDA-MB-231 cell proliferation compared to MCF-7 cells. IC50 for embelin in MDA-MB-231 cells was â¼4.45 μM and 3.28 μM at 24 h and 96 h, respectively. In contrast, IC50 for embelin in MCF-7 cells was â¼6.04 μM and 4.51 μM at 24 h and 96 h, respectively. Embelin (50 μM) induced apoptosis and activated caspase 3 activity in both cell lines when exposed for 72 h. Treatment of MDA-MB-231 cells with embelin (10 μM) for 24 h resulted in significant differential expression of 27 genes commonly involved in breast cancer carcinogenesis.ConclusionsOur findings show that embelin inhibits cell proliferation, induces apoptosis and alters expression of breast cancer focused genes in MCF-7 and MDA-MB-231 cells. Based on RT2-PCR array analysis, embelin down-regulated expression of pivotal oncogenes. This knowledge could be beneficial in the development of effective embelin-based therapies for treating breast cancer.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Pharmacological Reports - Volume 68, Issue 3, June 2016, Pages 638-644
Journal: Pharmacological Reports - Volume 68, Issue 3, June 2016, Pages 638-644
نویسندگان
Priyank Shah, Ransford Djisam, Hamidah Damulira, Alice Aganze, Michael Danquah,