کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
2011068 | 1066995 | 2011 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Combinatory effects of PBDEs and 17β-estradiol on MCF-7 cell proliferation and apoptosis Combinatory effects of PBDEs and 17β-estradiol on MCF-7 cell proliferation and apoptosis](/preview/png/2011068.png)
In the present work, we analyzed whether polybrominated diphenyl ethers (PBDEs) (47,99,100 and 209) interfere with the effect of 17β-estradiol on the proliferation and apoptosis of the MCF-7 cell line. MCF-7 cells were cultured in DMEM without phenol red supplemented with 5% charcoal-treated fetal bovine serum for 3 days with 10 nM 17β-estradiol; with 0.1 µM, 0.5 µM or 1 µM of the tested PBDE congeners; or with both 17β-estradiol and a congener. Cell proliferation was determined by measuring BrdU incorporation, and cell apoptosis was measured by caspase-9 activity.No PBDE congener had an effect on basal cell proliferation, but they all significantly decreased basal caspase-9 activity. An additive anti-apoptotic activity and ability to induce cell proliferation was observed in the presence of 17β-estradiol.
Journal: Pharmacological Reports - Volume 63, Issue 1, January–February 2011, Pages 189–194